Sequences from confirmation amplicon


Confirmation sequences for line 457F03 and hit At5g63070 are shown.
Line ID 457F03
Locus At5g63070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25299915
Primers used for PCR 54yz - 8474, show primer details
   
Sequence Name 05-K123234-0022-457-F03C-54yz
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 54yz: 5'-GTGATTAACCAACCTACGCAGACT-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K123234-0022-457-F03C-54yz
TTATTGTTTCTTTTAATTATGGATTGTATTCCCACTCTTCACGACATTTTCTTTATTATC
TTTTGCGTATTTAGGATACCAATGGAAGGAAATTGGCGGAGATTTGTGTTCTTCAAGAAC
TAGATTGAGACCTCTAACACGAACCGTTACTGCGGTGGTGTTCGAGTGTTAGAAGACCAG
ATATGACGTTACGTGCTTTGTAGATTTGCTACGACAAATGTTATTGTATGCTGCATCGAT
CTTAAAAGTTGAGTTTTATTTAAAGCCTTAAGGGACATTCTAAAGAAGAGAAGATAGAAA
TCACAAAGCACAATAAAATGGTCCAAAAAAAACAGAACAGAAAAAATCTTTCTGACTTGA
AAAAACAGAGCTTTTTCTTCATCACCTGAGAGGAATAAACCTGGTGGACCGACGGAAACA
ACAACAACCACAAATACGTGGTCTATGGTGATTAACCTTCTTGCAAGTCATCGAAAACTC
CGCAAGATAATGACCAATCATCTCAGGTTTAATCACGATTTCATTAAACTTCTTTCCATT
GTGCACTCCGACAACACTTCCAATCATCTCAGGGACAATGATCATGTTCCTTAGATGTGT
TTTCACCACTTCAGGCTTCATCTTGTTCTCATCGCTTGCTTCTTTCTTCGCCCTTCGTAG
CTTCTTGATCAGAATCAGAGGTTGCTTCTTTAATCCTCTGTAGAATCCTCTGCGAACACG
AGCGTTGAAGAGCTTAGCGAGATCAACGTTTGACATTTTGAGCAGAGCATCAACGTTGAA
CCCTCTGAATGAAAATTTCTTGAA
   
Sequence Name 01-K123233-0022-457-F03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K123233-0022-457-F03C-8409
GCCATTTACAATTGAATATTGATAATATTTCAAGATTAGATTACATGGNTTGGTAACAGC
CATAATTAGATTACAAGATTAGTAACATTAAATAATTGATAATATTTCAAAAATATTTAC
ATTTACTCGGTCAATCTTTAACATTTAGGTAAGTTTTCGATATTTCCGACGATCCTAGAG
ATTTACGATATCTCCCAACATTTTTGTATAAATAGTAGTGAAGCAAACAAAGAATTCTCA
CTACACCAATCTCTAATGGCATATTCTATCCTTTTGCAGAAAAACTCAAATTCCAACAAT
GGCGATCAACGAACCAGAGTTTGCCACGGCCGTGGCGGTGGCAACAAAGAAGAGAACGTT
CAAGAAATTCTCATTCAGAGGGTTCAACGTTGATGCTCTGCTCAAAATGTCAAACGTTGA
TCTCGCTAAGCTCTTCAACGCTCGTGTTCGCAGAAGATTCTACAGAGGATTAAAGAAGCA
ACCTCTGATTCTGATCAAGAAGCTACGAAGGGCGAAGAAAGAAGCAAGCGATGAGAACAA
GATGAAGCCTGAAGTGGTGAAAACACATCTAAGGAACATGATCATTGTCCCTGAGATGAT
TGGAAGTGTTGTCGGAGTGCACAATGGAAAGAAGTTTAATGAAATCGTGATTAAACCTGA
GATGATTGGTCATTATCTTGCGGAGTTTTCGATGACTTGCAAGAAGGTTAATCACCATAG
ACCACGTATTTGTGGTTGTTGTTGTTTCCGTCGGTCCACCAGGTTTATTCCTCTCAGGTG
ATGAAGAAAAAGCTCTGGTTTTTCAGTCAGAAAGATTTTTTCTGTTCTGTTTTCTTTGGA
CCATTTTTA

Last Updated on 06.12.2018 16:11