Sequences from confirmation amplicon


Confirmation sequences for line 400G01 and hit At5g67340 are shown.
Line ID 400G01
Locus At5g67340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26866436
Primers used for PCR 33sj - 8474, show primer details
   
Sequence Name 48-K116249-0022-400-G01C-33sj
GenBank Accession LN506611 [GenBank]
Sequencing primer gene specific primer, 33sj: 5'-GACAAGGCAGGCTTTATCTCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116249-0022-400-G01C-33sj
GCTTTTCTTGCAGTTGGTGTGAAACTAACAATGTCTATCCTCCTGATCCATTGGAGTTGA
TTCACTCAAGTGAGCCATTCCCTCTTCTTGTTGAATCAGTGAGAGCTTCATCATCAGAGA
ATGGCCATTCAGAATCTTTAGATGCAGAGGAACTGCGTCAGGTCTTTAGTAGGTCTGCTT
CGGCGCCAGGCATTGTCTCTGAAGTGGTTTGCAAAACCAAAAGAAACAACAATGCTGCTG
CAGATAGATCACTGACACGGAGTAATACCCCTTGGAAATTTCCAGAAGAGAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCA
   
Sequence Name 24-K116248-0022-400-G01C-8409
GenBank Accession LN497140 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K116248-0022-400-G01C-8409
ATCGGACATGAGCCATTTCATTGAATATATCCTCTCTTCTGGAATTTCCAAGGGGTATTA
CTCCGTGTCAGTGATCTATCTGCAGCAGCATTGTTGTTTCTTTTGGTTTTGCAAACCACT
TCAGAGACAATGCCTGGCGCCGAAGCAGACCTACTAAAGACCTGACGCAGTTCCTCTGCA
TCTAAAGATTCTGAATGGCCATTCTCTGATGATGAAGCTCTCACTGATTCAACAAGAAGA
GGGAATGGCTCACTTGAGTGAATCAACTCCAATGGATCAGGAGGATAGACATTGTTAGTT
TCACACCAACTTGCAAGAAAAGCTCTGACAATGAAATTAGGTGTCAAAGTGGTATGAGAT
AAAGCCTGCCTTGTCA

Last Updated on 06.12.2018 16:11