Sequences from confirmation amplicon


Confirmation sequences for line 544E06 and hit At5g67360 are shown.
Line ID 544E06
Locus At5g67360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26873015
Primers used for PCR KM56 - 8474, show primer details
   
Sequence Name 11-K101267-0022-544-E06C-8409
GenBank Accession LN491042 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K101267-0022-544-E06C-8409
TTACAATTGAATATATTCAGGAGATGGTTTAACACCGACGACTGTTCCTAAGATTGAAAT
CGAAGCGGTGGGATTAGGATCAGTAGTCACGTAATGCCGGATTATATCACCGGCTTTTTC
ACCAACGGTGGTCGCCGGTAACAAATGAGCATCCGCAACAAGCTCTTCACCATTCGCCGC
AGTGTTAGCCAGAATCATTCCAACTCCACCTGCTGCTTTAACCACATCACCTTTCTGAAC
TCTAGCATTAATTCCTCTATCACACATCACAATCTTCCCCTTTACTTTCTCCGGGATCAA
AGTTCCGGTCATACAGAGATTACCATTAGTAGCATTACTAGCATTCCCAGCGTAAATAA
   
Sequence Name 11-K101273-0022-544-E06C-KM56
GenBank Accession LN491043 [GenBank]
Sequencing primer gene specific primer, KM56: 5'-TTTATTTACGCTGGGAATGCTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K101273-0022-544-E06C-KM56
GGACTTTGATCCCGGAGAAGTAAAGGGGAAGATTGTGATGTGTGATAGAGGAATTAATGC
TAGAGTTCAGAAAGGTGATGTGGTTAAAGCAGCAGGTGGAGTTGGAATGATTCTGGCTAA
CACTGCGGCGAATGGTGAAGAGCTTGTTGCGGATGCTCATTTGTTACCGGCGACCACCGT
TGGTGAAAAAGCCGGTGATATAATCCGGCATTACGTGACTACTGATCCTAATCCCACCGC
TTCGATTTCAATCTTAGGAACAGTCGTCGGTGTTAAACCATCTCCTGAATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATT

Last Updated on 06.12.2018 16:11