Sequences from confirmation amplicon


Confirmation sequences for line 137E09 and hit F12F1 are shown.
Line ID 137E09
Locus F12F1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4016690
Primers used for PCR 21pm - 3144, show primer details
   
Sequence Name 47-K115064-0022-137-E09C-21pm
GenBank Accession LN506286 [GenBank]
Sequencing primer gene specific primer, 21pm: 5'-GCATAAGAAGGAAGAACATGGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K115064-0022-137-E09C-21pm
AGGCCAGAGGTTTAGATGAAGTTAAAACTTGATGGAAGAAATTACGTCCCTTCGGAAAAG
GCAAGAGCAGCTGGACTCTGAGCTTGAGGCAAGGTCTAAAGAAATCCGTACCGAGAAAAC
TAGTGCGGTTGCTCTACAGAAGCAATCTGAAGGGTTCCTTATTGAGTTTAACCGGTTGCT
TGAGGAAAACCAGGTGCTGCGAGACCAATTGCACACTGTGGATTCGAAACTTTCGCGTTC
AAGCAGCAAAAAGAATACTTGAGAGACCTTATTCCAACACTTGAAACTTCTTTGCGATTC
TGCCGTCTGAATATATATGTTTCGAGTTTGATATCAGGAGCTTGTGGTTTGGTGTTATCG
CATCTTGTTAACATGGAAGATGATTGGGAAAGATCAGAGAATACAAAACAAGTATAGGTT
TGCTTCTTGTTTAAGTGTTTAGCTAAGGTAGAAATTAATCAATTCTTCTGCTATAGGAAA
CTGTAACTCGTTGTAACTTGTTCATTTCGCAACAAAAACTTCTCGAATTGGTTCTTATTT
CCTTCCATATACGTATACAGTCGCTGCAAGTGGGTTGTCATTATAATTTCTCATAAGTTC
ACAGACTTGTTCAAAATAGATACTTGTTCCGAAATTCAGGAAGGATGCTGCAAGCTTCGT
GTTGGGGAATTTACCTCGCTCTTGACGAATTATTAAGGTTTTAGAAAATTGGGTCTCANA
CAGTCTATCATACATTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTG
TCCTCTCCNAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGG
ATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 15-K115063-0022-137-E09C-3144
GenBank Accession LN493096 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K115063-0022-137-E09C-3144
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAATGTATGATAGACTGTTTGAGACCCAA
TTTTCTAAAACCTTAATAATTCGTCAAGAGCGAGGTAAATTCCCCAACACGAAGCTTGCA
GCATCCTTCCTGAATTTCGGAACAAGTATCTATTTTGAACAAGTCTGTGAACTTATGAGA
AATTATAATGACAACCCACTTGCAGCGACTGTATACGTATATGGAAGGAAATAAGAACCA
ATTCGAGAAGTTTTTGTTGCGAAATGAACAAGTTACAACGAGTTACAGTTTCCTATAGCA
GAAGAATTGATTAATTTCTACCTTAGCTAAACACTTAAACAAGAAGCAAACCTATACTTG
TTTTGTATTCTCTGATCTTTCCCAATCATCTTCCATGTTAACAAGATGCGATAACACCAA
ACCACAAGCTCCTGATATCAAACTCGAAACATATATATTCAGACGGCAGAATCGCAAAGA
AGTTTCAAGTGTTGGAATAAGGTCTCTCAAGTATTCTTTTTGCTGCTTGAACGCGAAAGT
TTCGAATCCACAGTGTGCAATTGGTCTCGCAGCACCTGGTTTTCCTCAAGCAACCGGTTA
AACTCAATAAGGAACCCTTCAGATTGCTTCTGTAGAGCAACCGCACTAGTTTTCTCGGTA
CGGATTTCTTTAGACCTTGCCTCAAGCTCAGAGTCCAGCTGCTCTTGCCTTTTCCGAAGG
GACGTAATTTCTTCCATCAAGTTTTTAACTTCATCTAAACCTCTGGCCTTAACACCTTCC
AATAATGCTCCTTCCTTCTATAAACTTCCATGTTCT

Last Updated on 06.12.2018 16:11