Sequences from confirmation amplicon


Confirmation sequences for line 221G07 and hit F12F1 are shown.
Line ID 221G07
Locus F12F1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4027371
Primers used for PCR FA47 - 8474, show primer details
   
Sequence Name 39-K103433-0022-221-G07C-FA47
GenBank Accession LN503322 [GenBank]
Sequencing primer gene specific primer, FA47: 5'-TTCATTTGATCATTTGGCAATAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K103433-0022-221-G07C-FA47
ATGTTTTTGTGATTTGGTATATATCATCGATATTTGGAGATAAAAATTTCTCCAAAATAT
CCAGAGACAAAATAAGGAAATTAATTACTTTAAATAAGTAAAAGGGGAAATATCTAGATA
TTTTGCAAAGAATAACACCATGAGATATCCAAGTTTAATAATATCTAGAGACAAAGTAAA
AATAAATAATCTTTTAGCTAAAAGTGCAACTAGTATAGTAACGTCTTGATCGCATCCGTT
AGTGATTTTTTTTCTCTTCCTGATCCTGACAACACGATCAGTTAACTCAGAAAGCTCAAA
TGATTTATCTACTAATTGTTTTTAAGGATTTAATTATAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 55-K103434-0022-221-G07C-8409
GenBank Accession LN508781 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K103434-0022-221-G07C-8409
TTCGGACTGAGCCATTTATATTAAATCCTTAAAAACAATTAGTAGATAAATCATTTGAGC
TTTCTGAGTTAACTGATCGTGTTGTCAGGATCAGGAAGAGAAAAAAAATCACTAACGGAT
GCGATCAAGACGTTACTATACTAGTTGCACTTTTAGCTAAAAGATTATTTATTTTTACTT
TGTCTCTAGATATTATTAAACTTGGATATCTCATGGTGTTATTCTTTGCAAAATATCTAG
ATATTTCCCCTTTTACTTATTTAAAGTAATTAATTTCCTTATTTTGTCTCTGGATATTTT
GGAGAAATTTTTATCTCCAAATATCGATGATATATACCAAATCACAAAAACATCTATATA
TCTAGAGCTATATAGCTAAAAACAATTTATTGCCAAATGATTCAAATGA

Last Updated on 06.12.2018 16:11