Sequences from confirmation amplicon


Confirmation sequences for line 524H06 and hit F13C5 are shown.
Line ID 524H06
Locus F13C5 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10380356
Primers used for PCR BF09 - 8474, show primer details
   
Sequence Name 01-K028743-022-524-H06C-8409
GenBank Accession LN486440 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K028743-022-524-H06C-8409
GTACTAAGTTTCACAAAGACATAGAAAGTGTACTAGATAAGTTTCCCACGTAATAATCGA
TTATAAATATTAAATTATATATCGAATCAATAAAAGCAATAAATATTTAAAACATTAATT
TGACGTCGAATCATACATTTTGTGTTGGAGAAGGGATCACTTAAATGACACTACCTAATC
AAATTTAATGCATATTCTTTTGTTCCTTAATTTTTCTTCCTCGTCATTAAAAAAAATGGA
TACATTATATTACCTAGAATAAAATCAGTTCACAGAAAACAAAAGAATACTCTCTCTGTT
TTGAAATTATATATTCTGTAACATTCCATCCCAAAATTAGTATGACGTGATTAGTAGATC
AACAGACCACAA
   
Sequence Name 01-K028743-022-524-H06C-BF09
GenBank Accession LN486441 [GenBank]
Sequencing primer gene specific primer, BF09: 5'-AAAATTTTCAAATGGTTTGTGGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K028743-022-524-H06C-BF09
GATTTTATTCTAGGTAATATAATGTATCCATTTTTTTTAATGACGAGGAAGAAAAATTAA
GGAACAAAAGAATATGCATTAAATTTGATTAGGTAGTGTCATTTAAGTGATCCCTTCTCC
AACACAAAATGTATGATTCGACGTCAAATTAATGTTTTAAATATTTATTGCTTTTATTGA
TCATATATAATTTAATATTTATAATCATTATTACGTGGGAACTTATCTAGTACACATTCT
ATGTCTTTGTGAACTTAGTACACGAGTAACGGTTTTTCTATTAAATTAAAACCTTCAAAA
CAACATTTGATGAAAATTTCATAGATTTTACACCAAAATTTCGTAAGAAAAATTGTTGGA
GTTCGTATTGTTTTCTTGTAGTAGATGACATTAAAAAAATACATTAAACAAGAATTACCA
AAAGGCTTCATGTATCGTATTGTTTAGTGTATCAATTGTTTTCTTGTAGTATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAGAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11