Sequences from confirmation amplicon


Confirmation sequences for line 255C11 and hit F14J16 are shown.
Line ID 255C11
Locus F14J16 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20949486
Primers used for PCR 21bk - 8474, show primer details
   
Sequence Name 19-K107548-0022-255-C11C-21bk
GenBank Accession LN494787 [GenBank]
Sequencing primer gene specific primer, 21bk: 5'-GACAAGAGGTGGTCGATGATTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K107548-0022-255-C11C-21bk
GTATCGCAGACAGCTCATCGTCCTTCGGGTGAATCTGAATCCCGGAGGCAACTTTGCCTC
CACCATGCTTATACTACTTTCCTTCATCTCTTCTTCCGTCTCCATGATTTGATCCTTTTT
TTTTCTAGGTTTTAGTGTTTGATTAGTGGATGGTTAAGAGGAAAAGAGAGATAAAGAGAA
GAATAATTATTGGGATCATTTGGGTGTGTCCGAGGAGGAAGACCCTTGGTTTAAGTAGTA
ATTGCCCACCCAAAGCCGTCAACTTCTATATATATCATTTTTATAACTTTATACTATAAT
CATATGTTAGTATTTAATTTCCATTTCAAATCAATTATTAATTACGTACTCTATCCAAAA
CAATAATCATTTCCAAACAGACTCACATATTTTTGCCTAGACAGAATTAATAGCTGAGGA
TACACATAGATAATAATATGAAAAAGTATGATGCCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCC
   
Sequence Name 03-K107547-0022-255-C11C-8409
GenBank Accession LN487513 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K107547-0022-255-C11C-8409
TCGGGCTCAACTTTTTCTATTATTATCTATGTGTATCCTCAGCTATTAATTCTGTCTAGG
CAAAAATATGTGAGTCTGTTTGGAAATGATTATTGTTTTGGATAGAGTACGTAATTAATA
ATTGATTTGAAATGGAAATTAAATACTAACATATGATTATAGTATAAAGTTATAAAAATG
ATATATATAGAAGTTGACGGCTTTGGGTGGGCAATTACTACTTAAACCAAGGGTCTTCCT
CCTCGGACACACCCAAATGATCCCAATAATTATTCTTCTCTTTATCTCTCTTTTCCTCTT
AACCATCCACTAATCAAACACTAAAACCTAGAAAAAAAAAGGATCAAATCATGGAGACGG
AAGAAGAGATGAAGGAAAGTAGTATAAGCATGGTGGAGGCAAAGTTGCCTCCGGGATTCA
GATTTCACCCGAAGGACGATGAGCTTGTCTGCGATTACTTGATGAGACGATCGCTTCACA
ATAATCATCGACCACCTCTTGT

Last Updated on 06.12.2018 16:11