Sequences from confirmation amplicon


Confirmation sequences for line 129E08 and hit F14M4 are shown.
Line ID 129E08
Locus F14M4 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19288612
Primers used for PCR MS46 - 3144, show primer details
   
Sequence Name 16-K017404-022-129-E08C-MS46
GenBank Accession LN493213 [GenBank]
Sequencing primer gene specific primer, MS46: unknown
Sequence (A. th genome BLAST matches underlined) 
>16-K017404-022-129-E08C-ms46
GCCTAATTCATATCTTCTGATGCTAATCGGAGCAGATTTAACACTGGTCCGTATAGACGA
AGGGACTCCATAAACACCGTGTTCATCTGCAATGATCAATTGTAGTTAGGTCAAAAGTAA
AACCGTTTTCTTCAGCCGCAAGGAACTCTTTTGCAATTTGCATTTACCAGTTTTACCAAT
TTGCATTTACCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCG
GGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAG
GATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCT
TTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG
   
Sequence Name 20-K017404-022-129-E08C-3144
GenBank Accession LN495002 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K017404-022-129-E08C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTTTGGTAAATGCAAATTGGTAAAACTGGTAAATGCAAATTGCAAAAGAGT
TCCTTGCGGCTGAAGAAAACGGTTTTACTTTTGACCTAACTACAATTGATCATTGCAGAT
GAACACGGTGTTTATGGAGTCCCTTCGTCTATACGGACCAGTGTTAAATCTGCTCCGATT
AGCATCAGAAGATATGAAATTAGGAAACCTAGAGAT
Primers used for PCR 57xh - 3144, show primer details
   
Sequence Name 48-K119070-0022-129-E08C-57xh
GenBank Accession LN506656 [GenBank]
Sequencing primer gene specific primer, 57xh: 5'-AAGTCTCTTTAGCCCATGAAGGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K119070-0022-129-E08C-57xh
CGATAGGTTCAGTATCACGGGTAGATCGTACTGAGGTTGAAGAGTGAGGTGATCCGCCGG
CGCATGTTTATAATCGGCGGAGAGATTGAGCCGGAACCGTTGAAGAATCATGGCGAGAAC
GGTCTTGGCTTCCATTATCGCAAAGTTTTGGCCAATGCAAGCTCTTGGTCCCATTGAAAA
CGCGAGGAGAGCGTTCGGATGATTAGCGGCCCGAGAAAGACCGTTTGCAAACCGCATAGG
GTTGAATTTGTCGGCGTCACTTCCCCAAACGGCTTTGTCTCTGTGCATCTTCGCAATCGG
GAGGATTATAGTCGTGCCTTTAGGGATCTCTAGGTTTCCTAATTTCATATCTTCTGATGC
TAATCGGAGCAGATTTAACACTGGTCCGTATAGACGAAGGGACTCCATAAACACCGTGTT
CATCTGCAATGATCAATTGTAGTTAGGTCAAAAGTAAAACCGTTTTCTTCAGCCGCAAGG
AACTCTTTTGCAATTTGCATTTACCAGTTTTACCAATTTGCATTTACCAAACACTGATAG
TTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGA
AATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCC
CTTACATCAGTGGAGATAT
   
Sequence Name 16-K119069-0022-129-E08C-3144
GenBank Accession LN493645 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119069-0022-129-E08C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGTA
AATGCAAATTGGTAAAACTGGTAAATGCAAATTGCAAAAGAGTTCCTTGCGGCTGAAGAA
AACGGTTTTACTTTTGACCTAACTACAATTGATCATTGCAGATGAACACGGTGTTTATGG
AGTCCCTTCGTCTATACGGACCAGTGTTAAATCTGCTCCGATTAGCATCAGAAGATATGA
AATTAGGAAACCTAGAGATCCCTAAAGGCACGACTATAATCCTCCCGATTGCGAAGATGC
ACAGAGACAAAGCCGTTTGGGGAAGTGACGCCGACAAATTCAACCCTATGCGGTTTGCAA
ACGGTCTTTCTCGGGCCGCTAATCATCCGAACGCTCTCCTCGCGTTTTCAATGGGACCAA
GAGCTTGCATTGGCCAAAACTTTGCGATAATGGAAGCCAAGACCGTTCTCGCCATGATTC
TTCAACGGTTCCGGCTCAATCTCTCCGCCGATTATAAACATGCGCCGGCGGATCACCTCA
CTCTTCAACCTCAGTACGATCTACCTGTGATACTTGAACCTATCGACGGTTGAAGCAAAT
GCTTCTACACTACTCTGTATATGAACCTTCATGGG
Primers used for PCR 49xo - 8474, show primer details
   
Sequence Name 16-K119323-0022-129-E08C-49xo
GenBank Accession LN493647 [GenBank]
Sequencing primer gene specific primer, 49xo: 5'-AGAGACCTTTCTTTACTGGCAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K119323-0022-129-E08C-49xo
TCGCGAACAGATCTTATCGAACAAGTTTGTTTTTTTTTCTAAATCAAAGACAAAACCTGA
GATCCTTAAACTTTCTGGTAATGGACTTATCTTCGTAAATGGTCTTGATTGGGTTCGTCA
CAGACGAATCTTAAACCCTGCATTCTCTATGGACAAACTCAAGGTTCTTTAAAATCATTC
CTTCTTATATCTAATTAGTTGACAGTAGAAGATAGCACTTTGTTAACATTTCCTAAATTT
CTTCAGCTCATGACCCAATTGATGGTGGATTGCACTTTTAGAATGTTCCTTGAGTGGAAA
AAACAGAGGAATGGTGTAGAAACAGAGCAATTTGTGTTGATTAGCAGAGAGTTTAAGAGA
TTGACCGCTGATATCATAGCGACGGCTGCGTTTGGAAGCAGTTATGCGGAAGGAATTGAA
GTATTTAAATCACAATTAGAGCTCCAAAAGTGTTGTGCTGCTGCTCTTACTGACCTCTAT
TTCCCTGGAATCCAGTAAGTTTCAGTATAATCACTCTTTTGTTTTTAAACTTCGTTCTTG
TTTTAGTGATTTGAAAATTGGTTTTATTTCTTGTATCCCAAGGTACCTTCCTACGCCTTC
GAATCTTCAAATATGGAAGCTTGATATGAAAGTGAATAGCTCGATCAAAAGAATCATAGA
TGCGAGGTTAACATCGGAATCCAAAGATTACGGAAACGATCTTCTGGGAATCATGTTAAC
AGCTGCAAGCTCTAACGAGTCTGAGAAAAAGATGAGCATTGATGAGATCATAGAAGAATG
CAAGACGTTCTTCTTCGCAGGACACGAAACTACTGCGAATCTATTGACATGGAGTACAAT
GTTGCTTAGCTTGCACCAAGATTGGCAAGAGAAACTCAGAGAAGAAGTTTTCATGAATGC
GGTAA
   
Sequence Name 04-K119322-0022-129-E08C-oriL
GenBank Accession LN488134 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119322-0022-129-E08C-oriL
TTGAATTATCCTGAACATTCCAAAATTTTATACAGAAGACAAATTTACTAATCAGAAATT
ATGTTTGCAATCAACAATTATACAATACAAATGAAAACGGTTGAATATATTTGTTTAGCG
AAGGTTTGGAAAATTTGATTAAATGGTAACATATATAACCATGTGAATATATTTGTTTAG
GAAGGTTGCATCAAATAAAATATGTGGTGTTATGAACAAGTAACAGAGATATATCATTCT
CAAAATTAATTACACAATAAATGTTAAGAGAGTTAGTGGTGCAGGCAGGGATAGTCGTAT
GTGGCTTACACTTTTCTCCGTCACACCTCTTCAAACTTTATCACGTGGATCCTCAATTCC
GTATTTTATTGATTTTATTTCGTTTTCACTTGCGAAATTACTAATTTATATGCTGTTCTC
TTGTGCATTTTCATTGAAGAAATTTCTTCATCGAATCATGAATCAACGTGTAATGAGCAC
CGGACCGGACAAATAAGATGTTTACATCTCCTTGTAAACGAAGATCTTTCTTTTCTTTAC
TATACTGGTCACTTTCTTATTGAAAGACAATTACACTACATTGATATTGTTAATAAATCA
TTAGTAGGATGTGGATTTATCTGATGGTACATTGTTACCAAAATTCCTGATCAATCTGAC
CGTAGGGTCTTTTGACCAGTCGCTTTGACTTTACTCTTTGGCGCCTGATATCCGAACACG
TGTTACGTTAGTTAGCAATTGACGCCGTTAGTTGCCATCGTTAATGGGCTAGCCCAAATG
ATTCATAGTAATATCCCAATATCTTAATTAAGTCTCTTTAGCCCATGAAGGTTCATATAC
AGAGTAGTGTAGAAGCATTTGCTTCAACCGTCGATAGGTTCAAGTATCACAGGTAGATCG
TAC
Primers used for PCR 27xo - 8474, show primer details
   
Sequence Name 15-K119323-0022-129-E08C-27xo
GenBank Accession LN493203 [GenBank]
Sequencing primer gene specific primer, 27xo: 5'-CAAAGAGTAAAGTCAAAGCGACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K119323-0022-129-E08C-27xo
ATCAGGAATTTTGGTAACATGTACCATCAGATAAATCCACATCCTACTAATGATTTATTA
ACAATATCAATGTAGTGTAATTGTCTTTCAATAAGAAAGTGACCAGTATAGTAAAGAAAA
GAAAGATCTTCGTTTACAAGGAGATGTAAACATCTTATTTGTCCGGTCCGGTGCTCATTA
CACGTTGATTCATGATTCGATGAAGAAATTTCTTCAATGAAAATGCACAAGAGAACAGCA
TATAAATTAGTAATTTCGCAAGTGAAAACGAAATAAAATCAATAAAATACGGAATTGAGG
ATCCACGTGATAAAGTTTGAAGAGGTGTGACGGAGAAAAGTGTAAGCCACATACGACTAT
CCCTGCCTGCACCACTAACTCTCTTAACATTTATTGTGTAATTAATTTTGAGAATGATAT
ATCTCTGTTACTTGTTCATAACACCACATATTTTATTTGATGCAACCTTCCTAAACAAAT
ATATTCACATGGTTATATATGTTACCATTTAATCAAATTTTCCAAACCTTCGCTAAACAA
ATATATTCAACCGTTTTCATTTGTATTGTATAATTGTTGATTGCAAACATAATTTCTGAT
TAGTAAATTTGTCTTCTGTATAAAATTTTGGAATGTTCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCACAAATGTAGATT
   
Sequence Name 03-K119322-0022-129-E08C-8409
GenBank Accession LN487704 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K119322-0022-129-E08C-8409
TACATTGAATATATCCTGAACATTCCAAAATTTTATACAGAAGACAAATTTACTAATCAG
AAATTATGTTTGCAATCAACAATTATACAATACAAATGAAAACGGTTGAATATATTTGTT
TAGCGAAGGTTTGGAAAATTTGATTAAATGGTAACATATATAACCATGTGAATATATTTG
TTTAGGAAGGTTGCATCAAATAAAATATGTGGTGTTATGAACAAGTAACAGAGATATATC
ATTCTCAAAATTAATTACACAATAAATGTTAAGAGAGTTAGTGGTGCAGGCAGGGATAGT
CGTATGTGGCTTACACTTTTCTCCGTCACACCTCTTCAAACTTTATCACGTGGATCCTCA
ATTCCGTATTTTATTGATTTTATTTCGTTTTCACTTGCGAAATTACTAATTTATATGCTG
TTCTCTTGTGCATTTTCATTGAAGAAATTTCTTCATCGAATCATGAATCAACGTGTAATG
AGCACCGGACCGGACAAATAAGATGTTTACATCTCCTTGTAAACGAAGATCTTTCTTTTC
TTTACTATACTGGTCACTTTCTTATTGAAAGACAATTACACTACATTGATATTGTTAATA
AATCATTAGTAGGATGTGGATTTATCTGATGGTACATTGTTACCAAAATTCCTGATCAAT
CTGACCGTAGGGTCTTTTGACCAGTCGCTTG

Last Updated on 06.12.2018 16:11