Sequences from confirmation amplicon


Confirmation sequences for line 086B01 and hit F20D10 are shown.
Line ID 086B01
Locus F20D10 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17879901
Primers used for PCR 09lj - 8474, show primer details
   
Sequence Name 40-K114144-0022-086-B01C-09lj
GenBank Accession LN503784 [GenBank]
Sequencing primer gene specific primer, 09lj: 5'-CGAATGAATCTGTTTCTTGTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114144-0022-086-B01C-09lj
GCAGACAGAGTTGAAAGAAGTACCACACAAATGCAGGAAAAGCTAAGAACTTCAGAAGCA
GAGAAGACAGAGTTAGTTAAGGAAGTCGCGAGTTTGTCAACAGAAAAACGAAATCTTTTG
AGTTTTATCAGCGAAATGGAGGATGGAATGTTGAAGCTGTATGATGGAGACACGAAGCTG
ATGAAAACTTTGGAGAGAGTTACACAATGTTGTGATGGATTTGGTAAAGAGAACAATAAT
GGTGAAACTATTGGTTCTCCAAGATTGGCAATGAAGCATGAAGAAGATGTTGTAACTGAA
GACAGGTCACCATTTAGACCACTTAACCATTAGTTTGATTAATTCCACATAAGAGAGAGT
GAGAGAGAGTGAGAGAGAGAGAGAGAGGATATTTTTTTTTTCTCTTTGAAAACTTTGTTT
GTACTTTGTAGTGTTGATGTATCTGGAAGCTTTTTCTAAAACAAAGGTTTTGCTCATGTA
AAAAACAAACTTGTTAGGTTTTGCTTGCTAATTTTAATTATTTTCACAAATCCAAAACAA
CATAATTTAAAACTCAGGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAA
TATGGAGAAAAAAAAAGAGTATTTACCATTTTTTTTTCATTTCAAAAATGT
   
Sequence Name 13-K114143-0022-086-B01C-8409
GenBank Accession LN492130 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114143-0022-086-B01C-8409
ATTTATGTTGTTTTGGATTTGTGAAAATAATTAAAATTAGCAAGCAAAACCTAACAAGTT
TGTTTATTACATGAGCAAAACCTCTGTTCTAGAAAAAGCTTCAAGATACATCAACACTAC
AAAGTACAAACAAAGTTTTCAAAGAGAAAAAAAAAATATCCTCTCTCTCTCTCTCTCACA
CTCTCTCACTCTCTCTTATGGGGAATTAATCAAACTAGGGGTTAAGTGGTCTAAATGGTG
ACCTGTCTTCAGTTACAACATCTTCTTCATGCTTCATTGCCAATCTGGGAAAACCAATAT
TTTCACCATTATTGTTCTCTTTACCAAATCCATCACAACATTGTGTAACTCTCTCCAAAG
TTTTCATCAGCTTCGTGTCTCCATCAAACAGCTTCAACATTCCATCCTCCATTTCGCTGA
TAAAACTCAAAAAATTTCTTTTTTCTGTTGACAAACTCGCGACTTCCTAAACTAACTCTG
TCTTCTCTGCTTCAGAAGTTCTAAGCTTTTCCGGCATTTGTGTGGTTACTTCTTTCAACT
CTGTCTGCTTAGTTCCCAATTCGGCTCTGGACTTTGTTGCTTCTTGTCTGAAACAAAAAA
AGATTTCTTT

Last Updated on 06.12.2018 16:11