Sequences from confirmation amplicon


Confirmation sequences for line 088G03 and hit F22F7 are shown.
Line ID 088G03
Locus F22F7 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1601581
Primers used for PCR 22nj - 8474, show primer details
   
Sequence Name 07-K114479-0022-088-G03C-8409
GenBank Accession LN489374 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114479-0022-088-G03C-8409
ATTGGTATCTAATCACGTTTGTAGTGATAATCTAATCACGTTTGTTTTTAATGATAATGC
GTTGTCCCCATCCTTGTTCTTAATTCAATTGTCAATCACCTCCATTAATTTTGTGTTCAC
CTCGGTTAGAAAGCTTTGTTTTAATTTGTTAAGACGATGCTGATATATCCTGAATCATGA
TTAAATTAAAATCCTTAAGTTAAAGTCATTACAAAAACCACTTTTGAATATTGATCAGAT
ATTGGTCCCATGCCCAAAACGATGGTGCAAAATGTCTAAAGAGTGATAAGCTAGCAATTA
CTAGATATATACTAAAAGAAATAGTTTCAGTTCCAAGCATGAGCACTAGTCATAGTTGAG
GCTCACAAATTTCTTGCAAATCATCTTCGAGTTTTCCACGTCCAGAGATGGTCTCCTTCC
TCCTGATACACAANCAAACAA
   
Sequence Name 23-K114480-0022-088-G03C-22nj
GenBank Accession LN496654 [GenBank]
Sequencing primer gene specific primer, 22nj: 5'-GTTGTTTGTGTGTGTATCAGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114480-0022-088-G03C-22nj
TGATTTGCAGAATTTGTGAGCCTCAACTATGACTAGTGCTCATGCTTGGAACTGAAACTA
TTTCTTTTAGTATATATCTAGTAATTGCTAGCTTATCACTCTTTAGACATTTTGCACCAT
CGTTTTGGGCATGGGACCAATATCTGATCAATATTCAAAAGTGGTTTTTGTAATGACTTT
AACTTAAGGATTTTAATTTAATCATGATTCAGGATATATCAGCATCGTCTTAACAAATTA
AAACAAAGCTTTCTAACCGAGGTGAACACAAAATTAATGGAGGTGATTGACAATTGAATT
AAGAACAAGGATGGGGACAACGCATTATCATTAAAAACAAACGTGATTAGATTATCACTA
CAAACGTGATTAGATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11