Sequences from confirmation amplicon


Confirmation sequences for line 343D06 and hit F23H14 are shown.
Line ID 343D06
Locus F23H14 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:3297
Primers used for PCR 19qc - 8474, show primer details
   
Sequence Name 20-K115349-0022-343-D06C-19qc
GenBank Accession LN495343 [GenBank]
Sequencing primer gene specific primer, 19qc: 5'-CCAGACTCGAAAGAGCCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K115349-0022-343-D06C-19qc
AGATTGGGTATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC
CTCTCCGGAATCGAACCCTAATTCTCCGTCACCCGTTACCACCATGGTAGGCCACTATCC
TACCATCGAAAGTTGATAGGGCAGAAATTTGAATGATGCGTCGCCTGCACAGAGGCCATG
CGATCCGTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTA
TCTAATAAATGCGTCCCTTCCATAAGTCGGGGTTTGTTGCACGTATTAGCTCTAGAATTA
CTACGGTTATCCGAGTAGTAGTTACCATCAAACAAACTATAACTGATTTAATGAGCCATT
CGCAGTTTCACAGTCTGAATTCGTTCATACTTACACATGCATGGCTTAATCTTTGAGACA
AGCATATGACTACTGGCAGGATCAACCAGGTAGCATTCATCGATCACGGCAATTCCCCGC
CACATCCTCTCAAACGCAATGGAAAGAGAGAAAGGACGAGGTCTTGACCGTCATCTTTTG
CCCGAAGGACGGATGAGCTTTGGCGGGACTGAATCACTTCGAGTCACCGTCGACAACTTT
TCCGCATCCGAGATTTCAACCCGCAACTCATTCACCAATGCGAGCAAGGCAACAAATGCG
ATGACGAGAGGTGAACAAGCGCCGATGTTTGTGCCCACGACTTCCCCACGAAGGAGAAGC
CGCGGGCAAAGGTGTAAACCAAACTCAACAATTCCTTCAAATTAGGTACGAAACACAGGC
CCCGGAACTCATCATCGAGCGTAACATCGCCCGTGAATTAACTGAGAAGGTAGTTCGATG
GATCAGCAATGTAGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTTCATTCAAAATGTAG
   
Sequence Name 04-K115348-0022-343-D06C-8409
GenBank Accession LN488041 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115348-0022-343-D06C-8409
GCCATTTCATTGAATATATCCTGCCTACATTGCTGATCCATCGAACTACCTTCTCAGTTA
ATTCACGGGCGATGTTACGCTCGATGATGAGTTCCGGGGCCTGTGTTTCGTACCTAATTT
GAAGGAATTGTTGAGTTTGGTTTACACCTTTGCCCGCGGCTTCTCCTTCGTGGGGAAGTC
GTGGGCACAAACATCGGCGCTTGTTCACCTCTCGTCATCGCATTTGTTGCCTTGCTCGCA
TTGGTGAATGAGTTGCGGGTTGAAATCTCGGATGCGGAAAAGTTGTCGACGGTGACTCGA
AGTGATTCAGTCCCGCCAAAGCTCATCCGTCCTTCGGGCAAAAGATGACGGTCAAGACCT
CGTCCTTTCTCTCTTTCCATTGCGTTTGAGAGGATGTGGCGGGGAATTGCCGTGATCGAT
GAATGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGC
ATGTGTAAGTATGAACGAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT
AGTTTGTTTGATGGTAACTACTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGC
AACAAACCCCGACTTATGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTG
CCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCATGGCCTCTGTGCTGGCGACG
CATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGG
TAACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA
CATCCNAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACA
ATAAATAACAATACTGGGC
Primers used for PCR 75wv - 8474, show primer details
   
Sequence Name 66-K118684-0022-343-D06C-75wv
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 75wv: 5'-GGCTAGGAACTGGAACGAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K118684-0022-343-D06C-75wv
AAAACCCATTGTAGATGGATTTGCTTCTCGGAATTTTTGCTGCATCTCCAGTTACTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATAATACC
   
Sequence Name 54-K118683-0022-343-D06C-8409
GenBank Accession LN508634 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K118683-0022-343-D06C-8409
GCCTTTCATTGAATATATCCTGGAGCCTACGAACACTAGCTATCCGATCACCACTCATAC
GCCGAACGTTCAATTAACTCAAAATCATCAATCGTTCCAACTAATCTACCGAAGTACTCG
GCTAAGAAGAAAGAAGACGGACGAATCCGAGCCAAAGCCGTACAAGCGCGAGATACCTTC
GGGACAGCCTAGGCGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGA
CAAGGGTTCACATTTCGTTCATCACCCTTGGCCGGCTTTCGAACAGCCGGACTCCCATCA
AAAGATGGTTGCCAAGAACATCTTCGTTACGGTTTGCTAATTCTCGGAATAACATCAACA
AAACTAGGAAGGTTTAAAAAACCCACGGTAGTATGAAGTGCTACTCGAAATCTTTGCTAC
CCATCTCGTTCCAGTTCCTAGCCACAAGGTGTCGATGCCCATGGGCGACACTCACACCTC
GCTCACACTCGGGACAGCCTAGGCGGATCCATGCTTTCCAACGAAGCACGCCCATCCAAC
CTAGGCGAGACAAGGGTTCACATTTCGTTCATCACCCTTGGCCGGCTATCGAACAGCCGG
ACTCCCATCAAAAGATGGTTGCCAAGAACATCTTCGTTACGGTTTGCTAATTCTCGGAAT
AACATCAGCAAAACTCGGAAGGTTTAAAAAACCCACGGTAGTATGAAGTGCTACTCGAAA
TCTTTG

Last Updated on 06.12.2018 16:11