SimpleSearch - Line and FST details
Line specific information
| Line ID | 343D06 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N432874) |
| Segregation Analysis | 50:50:49 |
| Confirmed for Hit | At1g28130 F23H14 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 5772, 5774, 5849, 5856, 5858, 5863, 5866, 8745, 8750, 79082, 79105, 79117, 79147, 79154, 79166, 79167 |
| Note |
Gene hit At1g28130
| Sequence (A. th genome BLAST matches underlined) | >44-K016164-022-343-D06-8409 TGATCTGANATTTCCCGGACTGAAGCCATTTACATTGAATATATCCTGGAGCCTACGAAC ACTATGGGATCCTCCCTATAGTAGAGAGCCGAACGTTTAATTAACTCAAAATCTTCTGTC GTTCCAACTAGTCTACCGAAGTACTCGGGTGAGAAGAAAGAAGACGGACGAATCCGAGCC AGAGCCGTATCAGCGCGAGATACCTTCGGGACAGCCTATGGGATGCCTCTCGTATAGTGA GCTCCTGTAGACGTAATCCAAACAATCCTCAACCTCAGAGCAACAATCCTCCATTGTCTT GTCGTCAAGCTTTGGTGGGTCATTGCTGTGACGTGGCTTTAGCTCCCAGAAGAGCACGTA ATGCCCTGGGATTGATGACGTGTCTGCGTAGCTTGTGTACTCCGTGAGCAAGAGGCTTGA AGGATGTTGAAGATGGTTTAGTTTAGCTTGTGTCACTGCGTTTAGTAGATCTTCTTCGCT CGTTTTGTCAGTGTCGATGCTTAGTACAACGTTTCTTCGCTGCACGAAACGGAATTGAGG CGCTTTGTTGTGGAAACCCGTCACTTTTAGAATATCGCCTACTCTGTATCTGTACAAACC TATTGACAAAAACATATGTAACAGGTCACTTGTGCTACAAGTTACTATCCGTTTTAGGAA TCCACATCAGACCTATGG |
| GenBank Accession | AL952440 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:9827834 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F13K9 |
| Hit Gene Code | At1g28130 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Auxin-responsive GH3 family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit F21A14
| Sequence (A. th genome BLAST matches underlined) | >44-K016155-022-343-D06-8409 AGTGAGACTATGGGATACTCCCTATAGTGAGATTTAATGCATGATTAAAGTCTTGATTTT ATAAGTATAAGATCTTCATTTGTTAATTTGCTAATTGGGGTCTCTTTTTATGATTGTTAA GCTTTTGACTACTTTTGTAACAAACTTTCGGTCAAAGTCCTCTCATTTTGGGAATGGATA CTTTTGTGTCTCTTTCTTTATTATTCTTTGTCTCCCTCTCTTTTTCGAAAAGATTAAAAA AAATCGTACCAAACCCTTTACATAAAATCTATGGGATCCTCCCTATAGTGAGAAGCTCAT CCGTCCTTCTAGCAAAAGATGACGGTCAAGACCTCGTCCTTTCTCTCTTTCCATTGCGTT TGAGAGGATGTGGCGGGGAATTGCCGTGATCTATGAATGCTACCTGGTTGATCCTGCCAG CAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACGAGTTCAGAC TGTGAAACTGCGAATGGCTCGTTAAATCAGTTATACTTCGCTTGATGGGAACTACTACTC CGATAACCGTAGT |
| GenBank Accession | AL952439 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:14197279 - go to primer design |
| BLAST e Value | 2e-119 |
| Hit Clone Code (BAC ID) | F21A14 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
Gene hit F23H14
| Sequence (A. th genome BLAST matches underlined) | >54-K118683-0022-343-D06C-8409 GCCTTTCATTGAATATATCCTGGAGCCTACGAACACTAGCTATCCGATCACCACTCATAC GCCGAACGTTCAATTAACTCAAAATCATCAATCGTTCCAACTAATCTACCGAAGTACTCG GCTAAGAAGAAAGAAGACGGACGAATCCGAGCCAAAGCCGTACAAGCGCGAGATACCTTC GGGACAGCCTAGGCGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCTAGGCGAGA CAAGGGTTCACATTTCGTTCATCACCCTTGGCCGGCTTTCGAACAGCCGGACTCCCATCA AAAGATGGTTGCCAAGAACATCTTCGTTACGGTTTGCTAATTCTCGGAATAACATCAACA AAACTAGGAAGGTTTAAAAAACCCACGGTAGTATGAAGTGCTACTCGAAATCTTTGCTAC CCATCTCGTTCCAGTTCCTAGCCACAAGGTGTCGATGCCCATGGGCGACACTCACACCTC GCTCACACTCGGGACAGCCTAGGCGGATCCATGCTTTCCAACGAAGCACGCCCATCCAAC CTAGGCGAGACAAGGGTTCACATTTCGTTCATCACCCTTGGCCGGCTATCGAACAGCCGG ACTCCCATCAAAAGATGGTTGCCAAGAACATCTTCGTTACGGTTTGCTAATTCTCGGAAT AACATCAGCAAAACTCGGAAGGTTTAAAAAACCCACGGTAGTATGAAGTGCTACTCGAAA TCTTTG |
| GenBank Accession | HG968906 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:3297 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F23H14 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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