Sequences from confirmation amplicon


Confirmation sequences for line 304E12 and hit F2O15 are shown.
Line ID 304E12
Locus F2O15 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24002538
Primers used for PCR 32pj - 8474, show primer details
   
Sequence Name 41-K115005-0022-304-E12C-32pj
GenBank Accession LN504185 [GenBank]
Sequencing primer gene specific primer, 32pj: 5'-CCCTACTATGCAGGGACAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K115005-0022-304-E12C-32pj
AATAAGCAGACAGCATGAATATACAACAAATTTATTATCATTGACTGAAGAATATACAAA
GATTAAGAAAAAGATATAAAGAAGGTACAACTTTTCTACCCAATGAATCAATTGCGATAG
ACAGTAACTAACAAATCAAGAGTTTAGAAATATAAGAGAGTATAAGTACGAAAATTATGC
TGGGTATATACATGTCCGCTTATTTCATCATTAGCTCCAACCAATTGTAATGTGTTCTTC
TTCTCATCATCAGTAATTCAGTTTACAAACATTCGTTGACACCCAAAGCTTGGAAGTCTA
AAAAAAAATGTAAATGGCGCACAAGTAAGTAAATACTTAACGCAAACGCTGCCTTTGAAA
CAATATCAAAGATATTGCAGATATAAAGAAGTAAAATAAGAGATGACTTTAAAATTGAAG
TATTTGTATTAATACAAAAATCTTGCGTGAAAATACAATTGCGGTTTAATACAAAAAAGA
AATTGCAGATATAAAGAAGTAAAATAAGAGATGAAAGAAGAATAGTAAAAAGTATGAGAA
TTAATTTACCATTAAAAAAACACTTGAGCTTCGATTAAGATATTAAACTCACCCTTGTTT
TAAGGCAACTGTTCAAATGAGAAGCCAAAATTTGCCGTTGTTCCTTGAATGTTTGTGAGA
CGGGAGAATTATAGGCATTGATTGTATAAAAAAATAATCCAATGGAAAAATGTAAATGTC
CGCAG

Last Updated on 06.12.2018 16:11