SimpleSearch - Line and FST details
Line specific information
| Line ID | 304E12 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N429148) |
| Segregation Analysis | 50:46:30 |
| Confirmed for Hit | F2O15 At3g63470 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 73750, 73770, 73787, 73805, 73863, 73940 |
| Note |
Gene hit At2g04900
| Sequence (A. th genome BLAST matches underlined) | >93-K015592-022-304-E12-8409 CTGATCCATGGTAGATTTCCCGGACATGAAGCACTTTACAATTGAATATATCCTGCGGAC CACTCGGAGGCTGATGACTACATGTTTTATTTGCTGGAGGATTCGTGAAACTTGACAATA CTTCCTATGGTATCCTCCCTATAGTGAGTGTTTTTTTGTTGTTGTCCAGGTGTGGCAAAC GGCTTCACTTTACCATTTGGTTCACACTGCTGCTCTTGTTTCTGCTCCTATGGGATCCTC CCTATAGTGAGAGATGNTTGTCGGAGAGAGATAGTCCCCTGATACTACTTACTGGCGTCA TTCATTTCTTTCAACACTTTATTTTGCTTTAGTTCACTATGGGATCCTCCCTATAGGGAG AATCTGAGGCCGCACAGATTCAAAAAAGGCTGAAACTTGCTGATTAGAGACCAAAATGAA CTCACAGGAGTACCAATACCGGTTGTTTTGTATATACTAAACCCTCTCGAGATCGACTCA ACCATTACTAC |
| GenBank Accession | AL947467 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:1722714 - go to primer design |
| BLAST e Value | 5e-31 |
| Hit Clone Code (BAC ID) | F28I8 |
| Hit Gene Code | At2g04900 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hypothetical protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit F2O15
| Sequence (A. th genome BLAST matches underlined) | >41-K115005-0022-304-E12C-8409 CTGCGGACATTTACATTTTTCCATTGGATTATTTTTTTATACAATCAATGCCTATAATTC TCCCGTCTCACAAACATTCAAGGAACAACGGCAAATTTTGGCTTCTCATTTGAACAGTTG CCTTAAAACAAGGGTGAGTTTAATATCTTAATCGAAGCTCAAGTGTTTTTTTAATGGTAA ATTAATTCTCATACTTTTTACTATTCTTCTTTCATCTCTTATTTTACTTCTTTATATCTG CAATTTCTTTTTTGTATTAAACCGCAATTGTATTTTCACGCAAGATTTTTGTATTAATAC AAATACTTCAATTTTAAAGTCATCTCTTATTTTACTTCTTTATATCTGCAATATCTTTGA TATTGTTTCAAAGGCAGCGTTTGCGTTAAGTATTTACTTACTTGTGCGCCATTTACATTT TTTTTTAGACTTCCAAGCTTTGGGTGTCAACGAATGTTTGTAAACTGAATTACTGATGAT GAGAAGAAGAACACATTACAATTGGTTGGAGCTAATGATGAAATAAGCGGACATGTATAT ACCCAGCATAATTTTCGTACTTATACTCTCTTATATTTCTAAACTCTTGATTTGTTAGTT ACTGTCTATCGCAATTGATTCATTGGGTAGAAAAGTTGTACCTTCTTTATATCTTTTTCT TAATCTTTGTATATTCTTCAGTCAATGATAATAAATTTGTTGTATATTCATGCTGTCTGC TTATT |
| GenBank Accession | LN713415 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:24002538 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | F2O15 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
Gene hit At3g63470
| Sequence (A. th genome BLAST matches underlined) | >93-K015570-022-304-E12-8409 TGGTGCGCCGATTCATGCGGCGATGAAGAGAATCAGATGATTATGTACTCGATCATTTGG TAAACAGGTATTGGGCTATGGGATCCTACCTATAGTGAGATGTATGTACATGGAATTAAA ATGCTATGTGCATCATATATTCATATTCACCATTAGACGTTAATACTAAGCTAATCAGTG AATTAGCTGTCACAAAATCGAGAATTTGATTACAGAAATATGGGTGACTAATAATACATA CATATGCATAAAGTGTGTTGGAAATAATTAACAGGACCGGGATGCTCGTCACTGGCGTAT GGAGCATTACAAGAGCTTGGGCCGTTCAGAGTACACAGCGACGGCAAAACGCTTTTTAGA AACCGATATGCATGGAACAATGGTACCTATCGTTTTTCTTTTTTATTAAATAACAAAAAT AAATTAATGGTAAACGATTCTTGTGATTAAAAAATTATCAAACGGGTGGTGGTGGTGCAG CTGCGAATGTCTTGTTCTTAGAATCGCCGGCGGGAGTGGGGTTCTCGTACACCAACACGA CGTCGGACCTGGAGAAGCACGGNGACAGGAATACCGCAGCTGATAACTACATCTTCCTGG TTAACTGGCTGGGAGAGGTTCCCGGAGTACAAAGGCAGGGACTTGTACATCGGCGGTGAG AGCTATGCCGGGCACTATGTTTCCGAACTTGCCATACTATCCNTCTCCATCACCGGGAGC TTCTCAATCTCAAGGGC |
| GenBank Accession | AL947466 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:23439383 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | MAA21 |
| Hit Gene Code | At3g63470 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | serine carboxypeptidase-like 40 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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