Sequences from confirmation amplicon


Confirmation sequences for line 055H10 and hit K16E1 are shown.
Line ID 055H10
Locus K16E1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17023237
Primers used for PCR MS69 - 8474, show primer details
   
Sequence Name 43-K017402-022-055-H10C-MS69
GenBank Accession LN504667 [GenBank]
Sequencing primer gene specific primer, MS69: 5'-TCAAGAATCAGATCTTCCAAACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K017402-022-055-H10C-ms69
CAAGCTGTCGTTAATTAAAGGCTAATACTGCATCCCTCGGCTCCTGTCTTGTTAAGGGTA
TTTGGAGAAAGTTGCGAGGTCAAAGAGTTCTACGTACCGGAGAAAACAACACTTGTTGTT
AATCTCTATGCTGTGAATAGAGATCCTGATTCTTGGGAAGATCCTGATATGTTTAAGCCA
GAGAGGTTTTTAGTTTCTTCGATATCAGGAGATGAAGAAAAGATAAGAGAGCAAGCCGTG
AAGTATGTTACTTTTGGAGGTGGAAGGAGGACATGTCCCGCGGTAAAACTAGCTCATATC
TTTATGGAAACTGCGATTGGAGCGATGGTGCAGTGTTTTGACTGGAGAATTAAAGGAGAG
AAAGTATACATGGAAGAGGCTGTTTCAGGACTTAGTTTGAAAATGGCTCATCCGCTTAAG
TGCACTCCTGTTGTTCGGTTCGACCCTTTCAGCTTTTGATTTACATAATCCAAGTTCGGT
GACTTGTTAAGATCAAAGACGTTTTACTATGTTAGTTGTGCTTGTTGTATTTCAAAGTCA
AGTATGTAAGAATATTTTATGTTTATATGTAAGTTATGTCTAATAAAATAATAAAGATCA
CGTTCATATTAGCGTTAATGATTCTAAATTTCTAATATAAAAGAGGACATCATAAAATAA
CATTCGGATGTATTAATAACCATCATCCTTTGCTTGGATGTACTGCCTATCGATGCGCAA
GCAGGTTTTGCACAACAGAATGTCGCTGAAAGCACTTCCAGACTATGACTATATGTGAGT
CTTCAGCATCTCTCAGTCACTTGGATGATGC
   
Sequence Name 44-K017402-022-055-H10C-8409
GenBank Accession LN505026 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K017402-022-055-H10C-8409
CGGACATGAAGCCATTTACAATTGAATATATCATTTTCGTTTTGGGGTTTTTTGACTCAG
AGATGCTTGAAAACTGATAGACAACCTTATAGGTGCTTTGAGCATCATCCAAGTGACTGA
GAGATGCTTGAAGACTCACATATAGTCATAGTCTTGGAAGTGCTTTCAGCGACATTCTGT
TGTGCAAAACCTGCTTGCGCATCGATAGGCAGTACATCCAAGCAAAGGATGATGGTTATT
AATACATCCGAATGTTATTTTATGATGTCCTCTTTTATATTAGAAATTTAGAATCATTAA
CGCTAATATGAACGTGATCTTTATTATTTTATTAGACATAACTTACATATAAACATAAAA
TATTCTTACATACTTGACTTTGAAATACAACAAGCACAACTAACATAGTAAAACGTCTTT
GATCTTAACAAGTCACCGAACTTGGATTATGTAAATCAAAAGCTGAAAGGGTCGAACCGA
ACAACAGGAGTGCACTTAAGCGGATGAGCCATTTTCAAACTAAGTCCTGAAACAGCCTCT
TCCATGTATACTTTCTCTCCTTTAATTCTCCAGTCAAAACACTGCACCATCGCTCCAATC
GCAGTTTCCATAAAGATATGAGCTAGTTTTACCGCGGGACATGTCCTCCTTCCACCTCCA
AAAGTAACATACTTCACGGCTTGCTCTCTTATCTTTTCTTCATCTCCTGATATCGAAGAA
ACTAAAAACCTCTCTGGCTTAAACATATCAGGATCTTCCCAAGAATCAGGATCTCTATTC
CAGCA
Primers used for PCR 41xa - 8474, show primer details
   
Sequence Name 22-K118804-0022-055-H10C-41xa
GenBank Accession LN496327 [GenBank]
Sequencing primer gene specific primer, 41xa: 5'-GTGTAAAGGAGGTGGATCATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118804-0022-055-H10C-41xa
AAAATGAGAGAGAACGTGTTGTTTGTTGTGTCGGTCAATGGAAATAAAATGAAAAAGCGT
TACTTTATTGATGAGCAATACATGACACGTCATCTTACTTTTTATAAGAACTATTTATTT
ATTGCGGTTAATACACGTATTTGGAAAGTCATGTCGGCAACCATGAAATAATGAAGAAAT
TAACTTATTTGATTTCTCGAAGGAGCAAAGAAGAGTAGAAGACCAACACTTTTGGAGTGT
GGAGTGTGGAGTGTACTTTTGTTGGCAACATATGATGGAGATAGCTTGCCATGATTCCTA
GTAGGAGGCGGCTTGGATGTTGTCGGGCTGATAGAGAGACGCCTTGAGCATCGACTTACA
TTGGAAATGCTTTAACTAGTGAAAGACGAGATGAGAAGTGCCTTGAGTGGTAACAAATTG
ACTAGAAAGTGTTTTCGGAAATGACAATAAAGATGGGAGGTACTTTAGGCAATGACCTGA
CTAGGGTGGTTGAAGACCGACTAACGGTCTTGCAAAGGCCGTGTGCAAAAACTGCTCGTA
TAGAAGAAGGTGCTGGAGCATCTGACAAATAGCTCCAGAAATGCTTGAGGTATATTCAAT
TGTATGGAAGATGTGTGAGGTACTTTTGAGCAATACATGGCTGACTTAGGAATAAGGACC
AACATACAACATTAAATGTGCTTTAAGTAACATGTAGATGCATACATGGCATCAATTCCC
GGGAAATCTACATGTCCGGGTACCCTGTCCTCATACATGAACCGAAGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 03-K118803-0022-055-H10C-8409
GenBank Accession LN487695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K118803-0022-055-H10C-8409
TTCATGTATGAGGACAGGGTACCCGGACATGTAGATTTCCCGGGAATTGATGCCATGTAT
GCATCTACATGTTACTTAAAGCACATTTAATGTTGTATGTTGGTCCTTATTCCTAAGTCA
GCCATGTATTGCTCAAAAGTACCTCACACATCTTCCATACAATTGAATATACCTCAAGCA
TTTCTGGAGCTATTTGTCAGATGCTCCAGCACCTTCTTCTATACGAGCAGTTTTTGCACA
CGGCCTTTGCAAGACCGTTAGTCGGTCTTCAACCACCCTAGTCAGGTCATTGCCTAAAGT
ACCTCCCATCTTTATTGTCATTTCCGAAAACACTTTCTAGTCAATTTGTTACCACTCAAG
GCACTTCTCATCTCGTCTTTCACTAGTTAAAGCATTTCCAATGTAAGTCGATGCTCAAGG
CGTCTCTCTATCAGCCCGACAACATCCAAGCCGCCTCCTACTAGGAATCATGGCAAGCTA
TCTCCATCATATGTTGCCAACAAAAGTACACTCCACACTCCACACTCCAAAAGTGTTGGT
CTTCTACTCTTCTTTGCTCCTTCGAGAAATCAAATAAGTTAATTTCTTCATTATTTCATG
GTTGCCGACATGACTTTCCAAATACGTGTATTAACCGCAATAAATAAATAGTTCTTATAA
AAAGTAAGATGACGTGTCATGTATTGCTCATCAATAAAGTAACGCTTTTTCATTTTATTT
CCATTGACCGACACAACAAACAACACGTTCTCTCTCATTTTTCTTCTTCTCCGATCAATC
TAGGGTTTACTCTCACCGGCGACGAAAATGAT

Last Updated on 06.12.2018 16:11