Sequences from confirmation amplicon


Confirmation sequences for line 347E11 and hit MLM24 are shown.
Line ID 347E11
Locus MLM24 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8362946
Primers used for PCR 08wt - 8474, show primer details
   
Sequence Name 16-K118658-0022-347-E11C-08wt
GenBank Accession LN493638 [GenBank]
Sequencing primer gene specific primer, 08wt: 5'-TATGTACCATTTGGAAACCTCGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K118658-0022-347-E11C-08wt
TGTATTTCATATCTCTCATTGATCTTTCGAGTTAATAAAATTCCGTATGTAGGATTTTTT
TCTTTCGATCGATGGGTATTATCAAAACAATGGCTCTTGCGTATAATCTTCATGAGTTGA
GATACATGAATTTATGTAGTATATCTCCTACACAAGGCCAAAATTGCATGTGGCTAGATC
TAAAGGCTTCTCAATATTTCTCACGTATTTCTTGTATTGAGGATGTTCCCACAAAGTTTT
CAAAGTTTTGGGAGGTATTTTTTACCCATTCAAAAAAAAAAATTTCTTAACCATAAAAAA
TTCATTACCCCACTTGATTCAAATAGAATTTGGTTGTTTATTTTGGCTAAAAAATTTAAA
ATAACTAGTTATTATAAAATTTTTAAAACAATTTTTTATAGTATTTAACAACAACTCCAA
CAAATTCAATTGTAAATGGCTTCATGTCCGGAAAATCTACTTGGATCACCATTGAGTATA
ATGGTCAATATGGAAAAAAAAAAAAAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTA
A
   
Sequence Name 01-K118657-0022-347-E11C-8409
GenBank Accession LN486790 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118657-0022-347-E11C-8409
GCCATTTACATTGAATTTGTTGGAGTTGTTGTTAACTACTATAAAAACTCGTTTTAAAAA
TTCTATAATAACTAGTTATCTAATATCTATTAGCAAAAATAAACAACAAAATACTATTTG
AATCAAGTGGTGTAATGAATTTATTATGGTTAAGAACTTTTTTTTTTTGAAGGGGTAAAA
AATCCCCCCCAAAACTTTGAAAACTTTGGGGAAACATCCCCAATACAAAAAATCCGTGAA
AAATATTGAAAACCCTTTAAATCTACCCCCATGCAATTTTGGCCTTGTGTAGGAAATATC
CTACATAATTTCAGGTATCTCACCTCAGGAAAATTATCCCCAAAACCCTTTGTTTTGATA
ATCCCCATCGATCGAAAAAAAAAAACCCTACATCCGGAATTTTATTTAACTCGAAAGATC
ATTGAGAGATTTGAATTTCCAATCCAAAGATCCTTTTTTGGTAGGCTAAGGGTCAAGGTT
CCCAAATGGTACAAA

Last Updated on 06.12.2018 16:11