Sequences from confirmation amplicon


Confirmation sequences for line 055H10 and hit MNL12 are shown.
Line ID 055H10
Locus MNL12 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17376456
Primers used for PCR 11ok - 8474, show primer details
   
Sequence Name 17-K114734-0022-055-H10C-11ok
GenBank Accession LN493963 [GenBank]
Sequencing primer gene specific primer, 11ok: 5'-GACAGAGTTCTTTGCCTCTTCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114734-0022-055-H10C-11ok
ACATCCTTACAGAAGGATAGACCGTCAGGTGTTTGAAACAGATTATGCAGAGTTAGATGA
GATTGGTTTGCTCCGTACGTATTTCTATAATTTCTAACTCTTTCGTATTACTCGACTTTA
AAAGCCAACTGTTTAGCCAAGTGTTTCCAAATTCTTTTTTTTTGGATCTTTTTTCTTGTA
ACTATTTAGTACTTACCCTCGTGATTGGACACCTATTTAAGGAGAAGTTTTCTAATTAAC
ATGTGGTTAGAGAAAAAAAATCAAGTTAAAATTATATTTTCGTATTAAATTTATACTGTA
TATCATTTTTAATAGTCCAAACGTTATGTATGTATGCATTGGACATAGATATTTGTTTTT
TGACAAAGTAGTTAATGTAATGAAATAGCTTAAAAAATTACGAGCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 33-K114733-0022-055-H10C-8409
GenBank Accession LN501103 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K114733-0022-055-H10C-8409
GCATTTAAATTGCTCGTAATTTTTAAGCTATTTCATTACATTAACTACTTTGTCAAAAAA
CAAATATCTATGTCCAATGCATACATACATAACGTTTGGACTATTAAAAATGATATACAG
TATAAATTTAATACGAAAATATAATTTTAACTTGATTTTTTTTCTCTAACCACATGTTAA
TTAGAAAACTTCTCCTTAAATAGGTGTCCAATCACGAGGGTAAGTACTAAATAGTTACAA
GAAAAAAGATCCAAAAAAAAAGAATTTGGAAACACTTGGCTAAACAGTTGGCTTTTAAAG
TCGAGTAATACGAAAGAGTTAGAAATTATAGAAATACGTACGGAGCAAACCAATCTCATC
TAACTCTGCATAATCTGTTTCAAACACCTGACGGTCTATCCTTCTTGTAAGGATGTTATT
CATACCTCANATTAATGTTTCCATCTCCAAGTGAAGAGGC

Last Updated on 06.12.2018 16:11