Sequences from confirmation amplicon


Confirmation sequences for line 928E03 and hit MVI11 are shown.
Line ID 928E03
Locus MVI11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6673949
Primers used for PCR KN15 - 8474, show primer details
   
Sequence Name 67-K103226-0022-928-E03C-KN15
GenBank Accession LN511790 [GenBank]
Sequencing primer gene specific primer, KN15: 5'-TTTCCATGCACGATAAGATATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K103226-0022-928-E03C-KN15
TTTTTAGTGTTTTTATTATATATATATATATATATATATATATATATATATATACACACA
CACGTATTTAAATTTTGTGTCAACTCTATATATATATATAATCGCTCTTTCACCACATAT
TTTGTTCCCCTCTTAACCGCGTGTCAAAAACGTGGTTTTGAAAATTTGTGAGCGCACACA
ACACACAATAAAGACTTTGGAGAGTAACGTGTCTAAACGTGATAACTCACCTCTCTCTCC
CTAACAAAAAAAGTATATTTCTCTCTATATATATCACACACACACGCGCTCTCACATATA
AATATATTATGTACTAGAGATGTATTTAAAAAGAGATATTTGTCGCTGTTATTTAGGGGT
ATTTAATAGTGTGTGTGTGCACATGGGGAGTGAGGGGGGGAGAGAAAAGAATACATGATA
TATGGCTTCGTGTATCACTCATTTGTATATGGCTTCGTGTCCGGGATATCTACGTGGATC
AACAGTGAGTGTGAGGGTCTATATAGAGAAAAAGAGAGAGTATTCACCTTTTTTTTTTCT
ACTCAAAAATGTATATCTCCCCC
   
Sequence Name 70-K103227-0022-928-E03C-8409
GenBank Accession LN512367 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K103227-0022-928-E03C-8409
GCCATTTACATTGAGTTATACATGAAGCCATATATCATGTATTCCTTTCTCTCCTCCCTT
CACTTCCCATCTGCATACACACAATATTAAATACCATTAAATAACAGCAACAACTATCAT
TTATTAATTACATCATTAGTACATCATATATTCATATGTGAAAGCATTTGTTTGAGTTAT
ATATATAGAGATAAATGTACTTTATTAGTTAGGGATAGTGATGTGATTAATCATGTTTAG
ACAAGTTACTATCCAAAGTCTTTATTGTGTTTTGTGTACGATCATAAATTTTCAAAACCA
TGTTTTTGACACGTTGTTAAGATGGTAACAACTTATGTTGTAAATGATCGATTATACTAA
TATTAAGATTTGACAATTAATTTAATTACGTGTATGTTTATATATATATATATATATATA
TATATATATATATATATATAAACACACATAAAAAGTGTGTATATAATATAATATACCTTT
TTGTATATCTTAGCGCGCGGGAAAAAAA

Last Updated on 06.12.2018 16:11