Sequences from confirmation amplicon


Confirmation sequences for line 760E07 and hit T10O24 are shown.
Line ID 760E07
Locus T10O24 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3444656
Primers used for PCR 15do - 8474, show primer details
   
Sequence Name 44-K109938-0022-760-E07C-15do
GenBank Accession LN505214 [GenBank]
Sequencing primer gene specific primer, 15do: 5'-GGCCTATGCGAATATCATAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K109938-0022-760-E07C-15do
ATAGAGGAGAAGAATCCGTACAAGGAATCCAAAAAATAAAAATATACCATCGTGAGATAT
ATACAACTTCTTAGTCAAAATCTCGTCATTACTCATTAGTGTGATTGGAAAAAAAAAACT
GAAAATGGAATCACTCACTGTTAAGAATAATAAACCAGAAGATTAAAGAAGCTAATTTTG
GTCATACACCATTACAATTCATAGTAGCGTGTTTAAACTTAACCGGCTCGGTTCTTCGGA
TTGGTCTTATTTGTATTAAGTACTAACCACTAAGACAATTTTATATTGTTCCATGTGATT
GGTCAGTTTCTAATTTAAAATTTATGGTTCAAATGCAATTCATAGAATAATATTAAGATC
GGGTTTTGTGGGTTGACGAGAAAATTAGTATACGCCTAACTAGTAGTATATCCATCCATA
TGACTCTAGAAATTTTATGTTATCCAAACACTTTTATTTATATCGTTTTCAAATGGCAAA
AAAAAAAAANAAAAAAAGGGGAGGGGGGCCATTTTTTTTAAAAAAAAGGGGTTTTGCCCC
CCCCTTAAAATTTGGACCCCCCGGCCCCGGGGAAATCTAAAAAGGGGCCCCCTAAATTTT
TTTTTGGGAA
   
Sequence Name 36-K109937-0022-760-E07C-8409
GenBank Accession LN502260 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K109937-0022-760-E07C-8409
ATTGTTCCAAAAAATATTTATGGGTCCCTCTTTTAGATTTCCCTGGGTCCCGCGGATCCA
AATTTTAATGTGGACGTCAAATCCTTTTTTTTTATTATTATTTGTCCACTTTCCTCTTCT
TCTTTTTTTTTTTTTGGCCTTTGAAAAAAAAAAAAAAAAAAGGGGTGGGAAAAAAAAAAA
TTTTCAAGGGCAAAGGGGGGGGAAAACCAAAAGGTGGGGGGAAAAAAATTTTTCCGGCAA
CCCCAAAAACCCGAATTAAAAAATAATCCAAAAAAGGGCTTGGAACCAAAAATTTTAAAT
TAAAAACGGGCCAACCCCGGGGAAAAAAAAAAAATGGGCTAAGGGGGTAGGCCTTAAAAA
AAAAAAAAACAACCCAAAAAACCGGGCCGGGTAATTTTAAAACCCCCCCAAAAAATTGGA
AGGGGGGAAAAAAAAAAAAAACCTCCTAAAACCCCGGGGTTAAAAATCCAAAAAGGGGGG
GGACCCCTTTTCCGGTTTTTTTTCCCCCCCCCCCCAAGGGGAAAGGGGAAATTTTTTAAA
AAAAAATGGGAAATTTTCCCAAAGGGGAAATTTTTTTTTTTGGGAGACCCTTGGGAGGAA
TTCCTCCCCCCCTAATATTTTCCCAATTTGGAAAAAATATTTTCCCCAAAAA

Last Updated on 06.12.2018 16:11