Sequences from confirmation amplicon


Confirmation sequences for line 358A06 and hit T1B9 are shown.
Line ID 358A06
Locus T1B9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2260508
Primers used for PCR 15nz - 8474, show primer details
   
Sequence Name 05-K114625-0022-358-A06C-8409
GenBank Accession LN488454 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114625-0022-358-A06C-8409
CATTACATTGAATAATCCTGGTCAATTGATCCATATTGTCCAAGCATTCAAGTCCAAATG
GTCATCTAATAACTCACATTTGAAGCATAGTTGTCCATTGATAAGTGTCCAAGCACTCTA
GTCCAAGTGGTCATCTAGTAACTCACATCTGAAGCATAGTTGTCCATTGATGAGTGTCCA
AGTACTCAAGTTTAAGTATATAAAGCGTGTAAGAAGATCAATTGTAGCAGCAATGTTCTG
TTGTTGATTCTTAATATATTCTTCGTTTTTAAGTTATGATAGAGAATTTTGAGATAATCT
CAGAATTTCTATGATTTGTAAGAATTATAGAGAACCACCATGATTGATGATTTTGAAAAA
GAAAATAAAAATGATGAAAAAAAAAACATGGAAGACAGAGAAGATAAAAAATGATGCTAG
TTTGTCTCCATGATAACTTGTCCACGTAAACGTAACCATGTGGAAATAAGTGATTCTCAT
AAGCAAAAGAATATGCTTTTAAGGTTAGACGTTCAATGAAACATCAATTTATCAAGAAAG
AATTAAATTGTACCAAACAAGGCGATCAAATGGATATGAAATTTAATACTGGAATTTGCC
AACTTGGGTTTTCCATAAACTCTCAAATGTTTGATTTTGATAAACTCTTAAATGTTTGAT
TTTGCAAGGAAAGAAAAAAAAGTAATATTGAGAAGAGAGAGAGAGAAGAGAGAGAGAAGA
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGAAAAAGAGGGAA
Primers used for PCR 82wk - 8474, show primer details
   
Sequence Name 43-K118366-0022-358-A06C-82wk
GenBank Accession LN504998 [GenBank]
Sequencing primer gene specific primer, 82wk: 5'-ATAATCGGATTCAGTGATAAACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K118366-0022-358-A06C-82wk
GCGGATCTGGCATTACAGAGTATAAACGCTGAGCTCCGGCGAAGAAACGATAACGAATCA
AGTGATGACCAAAATCGGAGTCTGGTTTACTCTCGGTTTGGGATCTGTGAGTTTGTGATT
TTCGGTTTGTTCTGGTTTACTCTCGGTTTGAGATTTAAGGGGAATAAGGGATTGTATGCA
AGTGGGCTCCGAAAGGTTTGGGCTTCGTCAAGTTTGACAAACACAATCATTAAAAGGCAA
TATGACACGTTTAGGCACTTTTAGATAATTTTATGAGAATATTACACGTAGAGGTACTAT
TTTGAAACTTTAGCCACAAATAGGCACTTTGAAAGGAGAAGGCACTTTACCAAATCAAAA
TTCCCAAATTGCCCTCTGAACCACAACGCTTTCTCCTCATTTTCTCTCTCAACTCTTTTC
TCTATCGTCTTTATTTCTCTCTCTTTCCAAAATCAAAACCAATTTTTTACGGGAATCTCA
ATATTCCAAATCAAAACTAAAAACCCAATTTCATATGATTTATCATCATCTTCCCTGTCT
CTCAACAATTATTTGATTTGGTTCCATTTATTTCTCTCCTTGATTAAGGAATCTCTGTCA
TCGATTACAATGAAAAGTGAGGATGTTAACCATGATCAGTTACCTTCTAAGCAATCTGGG
CTTGAAACGGTTCGTGATGTTGAGAACGCGAGTAGCAGCAAAAAGGCCATTGTCGATGTA
AACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAA
AATGTAGATGTC
   
Sequence Name 64-K118365-0022-358-A06C-8409
GenBank Accession LN511293 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118365-0022-358-A06C-8409
TCATTGAATATATCCTGTTTACATCGACAATGGCCTTTTTGCTGCTACTCGCGTTCTCAA
CATCACGAACCGTTTCAAGCCCAGATTGCTTAGAAGGTAACTGATCATGGTTAACATCCT
CACTTTTCATTGTAATCGATGACAGAGATTCCTTAATCAAGGAGAGAAATAAATGGAACC
AAATCAAATAATTGTTGAGAGACAGGGAAGATGATGATAAATCATATGAAATTGGGTTTT
TAGTTTTGATTTGGAATATTGAGATTCCCGTAAAAAATTGGTTTTGATTTTGGAAAGAGA
GAGAAATAAAGACGATAGAGAAAAGAGTTGAGAGAGAAAATGAGGAGAAAGCGTTGTGGT
TCAGAGGGCAATTTGGGAATTTTGATTTGGTAAAGTGCCTTCTCCTTTCAAAGTGCCTAT
TTGTGGCTAAAGTTTCAAAATAGTACCTCTACGTGTAATATTCTCATAAAATTATCTAAA
AGTGCCTAAACGTGTCATATTGCCTTTTAATGATTGTGTTTGTCAAACTTGACGAAGCCC
AAACCTTTCGGAGCCCACTTGCATACAATCCCTTATTCCCCTTAAATCTCAAACCGAGAG
TAAACCAGAACAAACCGAAAATCACAAACTCACAGATCCCAAACCGAGAGTAAACCAGAC
TCCGATTTTGGTCATCACTTGATTCGTTATCGTTTCTTCGCCGGAGCTCAGCGTTTATAC
TCTGTAATGCCAGATCCGCGGTAACGCANGTCAGTGACCTACTCCGGCGTTTATCACTG

Last Updated on 06.12.2018 16:11