Sequences from confirmation amplicon


Confirmation sequences for line 001H07 and hit At4g18960 are shown.
Line ID 001H07
Locus At4g18960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10384371
Primers used for PCR MR27 - 8474, show primer details
   
Sequence Name 33-K015326-022-001-H07C-8409
GenBank Accession LN500781 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K015326-022-001-H07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCATATTATCATTAGTGGGA
ATATTTGTTTGATATGTTTATTTTATTTACTTCGGGGGTTTTTGTAACAATTTTGTAGAT
CTAATTTCTTGTTCTTCATGTGTATATTAATTTTCCCTTAAGACTTAAATAAAAAGAGAG
AATTTGTTATATATAGATATATGAAGTGAGGGAAATGGTACAAAGTTAAAGGAGATCTGA
GTGAGAGTTAGATAATAAATGAAAAGAAATAAGAAACCATCAGGGTTTTTTCTAATGTGG
AGTTTTAGATTCAGTTTTGTAGAACTAAGATTCACTTTGTTGGGTGTTCTTTCTTCACTC
ATTTCTGTTATTATAATAATAATAAAATCTTATATCTTTCTATTTTCCTTACTAACAAGT
ACTTGAAGATTTAGATATATTTATAGATCTGGTGTTGTAATAGGTAAAAACTTGATTTTT
ATGACTATAAAAGTAAGTTTTGGGAAACAAATTGGGGAGAGAGTAAGGAAGGACTATGAG
GTCATATCTTCTGTTTTGTGATCATCCATCCTCCATTGTT
   
Sequence Name 34-K015326-022-001-H07C-MR27
GenBank Accession LN501204 [GenBank]
Sequencing primer gene specific primer, MR27: 5'-ACAGACATTAACAACAATGGAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K015326-022-001-H07C-MR27
TGGATGATCACAAAACAGAAGATATGACCTCATAGTCCTTCCTTACTCTCTCCCCAATTT
GTTTCCCAAAACTTACTTTTATAGTCATAAAAATCAAGTTTTTACCTATTACAACACCAG
ATCTATAAATATATCTAAATCTTCAAGTACTTGTTAGTAAGGAAAATAGAAAGATATAAG
ATTTTATTATTATTATAATAACAGAAATGAGTGAAGAAAGAACACCCAACAAAGTGAATC
TTAGTTCTACAAAACTGAATCTAAAACTCCACATTAGAAAAAACCCTGATGGTTTCTTAT
TTCTTTTCATTTATTATCTAACTCTCACTCAGATCTCCTTTAACTTTGTACCATTTCCCT
CACTTCATATATCTATATATAACAAATTCTCTCTTTTTATTTAAGTCTTAAGGGAAAATT
AATATACACATGAAGAACAAGAAATTAGATCTACAAAATTGTTACAAAAACCCCCGAAGT
AAATAAAATAAACATATCAAACAAATATTCCCACTAATGATAATATGAATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11