Genotyping and primer details

Genotyping and primer details for 001H07 - At4g18960
Line ID 001H07
Locus At4g18960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10384371 of line 001H07 Chr4:10384371
Insertion allele amplicon 8474 - MR27
Locus specific primer MR27: 5'-ACAGACATTAACAACAATGGAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10384913
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11