Sequences from confirmation amplicon


Confirmation sequences for line 002A11 and hit At3g49680 are shown.
Line ID 002A11
Locus At3g49680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18424099
Primers used for PCR CD14 - 8474, show primer details
   
Sequence Name 10-K029909-022-002-A11C-8409
GenBank Accession LN490523 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K029909-022-002-A11C-8409
AACTGCTCAACGGTTGGANAGGCATACACATCCTCTCAGCACCATTTCTCATACGCTTTG
CATTCTCCTCAGGACGAAAGAGGAGGATGTTATTACCATCTTTCTTTCTGTAAGCTTTTA
GCCCTTCAAACAATCCCTAGAAGAACATACACTAATAAGACTCATGTGATCCTAAATTGC
ACTCTTTACCACAACACATTGCTTGGCATTTCTCATCATCACTTACAATATAAGAAGATT
TCTCCGTGCAATATATGAATATACATTCAGTAAATAAATCCTCACTAGATGCAACTAACA
TAACAACTAGGAGACTAGACTTATTGACAAACTCAGG
   
Sequence Name 10-K029909-022-002-A11C-CD14
GenBank Accession LN490524 [GenBank]
Sequencing primer gene specific primer, CD14: 5'-ACAATGTATCACAGCCTGAGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K029909-022-002-A11C-CD14
AGTTGCATCTAGTGAGGATTTATTTACTGAATGTATATTCATATATTGCACGGAGAAATC
TTCTTATATTGTAAGTGATGATGAGAAATGCCAAGCAATGTGTTGTGGTAAAGAGTGCAA
TTTAGGATCACATGAGTCTTATTAGTGTATGTTCTTCTAGGGATTGTTTGAAGGGCTAAA
AGCTTACAGAAAGAAAGATGGTAATAACATCCTCCTCTTTCGTCCTGAGGAGAATGCAAA
GCGTATGAGAAATGGTGCTGAGAGGATGTGTATGCCTGCTCCAACCGTTGAGCAGTTTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
C

Last Updated on 06.12.2018 16:11