Sequences from confirmation amplicon


Confirmation sequences for line 002D03 and hit At5g51400 are shown.
Line ID 002D03
Locus At5g51400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20879945
Primers used for PCR 02kw - 8474, show primer details
   
Sequence Name 17-K113982-0022-002-D03C-02kw
GenBank Accession LN493935 [GenBank]
Sequencing primer gene specific primer, 02kw: 5'-GGAAATTTCTTCTGCAGTTAAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K113982-0022-002-D03C-02kw
AGTCAACGGAAGAGAAGAAGACCAAATTAGTCCTTAAACTCGTTTAGGTAACAGTTTGGT
TTAAAGCACAGAAACAGCCCTTCAATAAGTTTCAATGTCATAAAACCAAAAAAAAAAAAA
TTGGGGAAAACAAAAACCTTTGGGGGACCCCTTGGGTTTCCCCAACCCTTCAAAGGGGTC
GAAAAAAACCCTTTTTCCCCCTTTTTCCCCCTTTTTCAATTCAATTTGGGGGGAACCAAT
TTGGGATTTCAAAAGGCGGAAGGGAAATTTCAATTGGAAAGGGGTTCCGGGCCGGGAAAT
CCCCCGGGGACCCCCAGGGATTTGGGGGGCAAATGGGGAAAAAAAAAAAGATAATTCCCC
ATTTTTTTTCAATTCAAAAAGGGAAATTTCCCCCGCGTTTTAAAAA
   
Sequence Name 01-K113981-0022-002-D03C-8409
GenBank Accession LN486657 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113981-0022-002-D03C-8409
ATTTACATTGAATATCTCCGTCCGACATCTGAAATCCAAAATTAGATCAGCCAAAATTGA
ATTGAAAAATGGAGAAAAAGAGAGAAAAAGAGATCTTTACGAACCATATGAAGGATTGGA
GAAACCGAAGAGTGTCCTCAAAGGTTCGTCGTCTTCACTATTTTTTTTTTTTTGGGTTTT
AGAACTTGAAACTTTTTGGAGGGGTTTTTTTTGGTTTTAAACCAAATTTTTTCCTAAAAG
ATTTTAAAAACAATTTGGGTTTTTTTCTTTCCGGTGGACCCCCCCACTTTTTAATTAACC
CCCCCCTTTTTTAAAAAAATTACTTTTAACTGGAAAAAAAATTTCAA

Last Updated on 06.12.2018 16:11