Sequences from confirmation amplicon


Confirmation sequences for line 002E11 and hit At5g61480 are shown.
Line ID 002E11
Locus At5g61480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24727061
Primers used for PCR KE49 - 8474, show primer details
   
Sequence Name 06-K101217-0022-002-E11C-8409
GenBank Accession LN488735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K101217-0022-002-E11C-8409
ATTTACATTGAATAATCCTGGTTCTCTGGTTCTCTGCCTCAAGAACTCGGGAATCTCTCA
AACCTAGAGACTTTATTTCTATTCCAAAACGGTTTCACCGGTGAAATCCCAGAGAGTTAT
AGCAACTTGAAATCCCTCAAGCTTCTCGATTTTTCGAGTAATCAGCTTTCTGGTAGTATC
CCATCAGGCTTCTCGACCTTGAAGAACCTCACATGGCTAAGCTTAATCAGCAATAACCTC
TCAGGTGAAGTACCTGAAGGAATCGGTGAACTCCCTGAGCTTACTACATTGTTTCTATGG
AACAATAACTTCACCGGAGTTTTGCCACACAAGCTTGGATCAAACGGTAAACTTGAGACA
ATGGACGTCTC
   
Sequence Name 06-K101223-0022-002-E11C-KE49
GenBank Accession LN488736 [GenBank]
Sequencing primer gene specific primer, KE49: 5'-AGAGACGTCCATTGTCTCAAGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K101223-0022-002-E11C-KE49
ACTCCGGTGAAGTTTTGTTCCATAGAAACATGTAGTAAGCTCAGGGAGTTCACCGATTCC
TTCAGGTACTTCACCTGAGAGGTTATTGCTGATTAAGCTTAGCCATGTGAGGTTCTTCAA
GGTCGAGAAGCCTGATGGGATACTACCAGAAAGCTGATTACTCGAAAAATCGAGAAGCTT
GAGGGATTTCAAGTTGCTATAACTCTCTGGGATTTCACCGGTGAAACCGTTTTGGAATAG
AAATAAAGTCTCTAGGTTTGAGAGATTCCCGAGTTCTTGAGGCAGAGAACCAGAGAACCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATATCCGC

Last Updated on 06.12.2018 16:11