Sequences from confirmation amplicon


Confirmation sequences for line 002E12 and hit At2g25312 are shown.
Line ID 002E12
Locus At2g25312 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10780065
Primers used for PCR 03kw - 8474, show primer details
   
Sequence Name 34-K113947-0022-002-E12C-03kw
GenBank Accession LN501494 [GenBank]
Sequencing primer gene specific primer, 03kw: 5'-AATGGGTGAGAGCATACAAAGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113947-0022-002-E12C-03kw
TTCTTGAGATACTAAAGGTAATAATGATTTTGCAATGGCTCCAATTTCAGAGAATGGGTT
GCTACCAAATCCAATGGCTTGTGTGAAAGATGCAGGAAAAATTCCAGATTGTGTAGAGGC
AATGAAACAAGGTTATTTAAAAGATATAACAAAAGAGTGTTGCTTTATTCTACTTAGTCT
TCCCGAAGATTGTTTTGGGATTCTATTTCCTATGCGTTTATACTATCGTATCGTACTTAA
GGTTACATGCAAATTATTAGGCATTTTTTAATCACAATTTATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTA
   
Sequence Name 06-K113946-0022-002-E12C-8409
GenBank Accession LN488898 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K113946-0022-002-E12C-8409
CATTTCATAAATTGTGATTAAAAATGCCTAATAATTTGCATGTAACCTTAAGTACGATAC
GATAGTATAAACGCATAGGAAATAGAATCCCAAAACAATCTTCGGGAAGACTAAGTAGAA
TAAAGCAACACTCTTTTGTTATATCTTTTAAATAACCTTGTTTCATTGCCTCTACACAAT
CTGGAATTTTTCCTGCATCTTTCACACAAGCCATTGGATTTGGTAGCAACCCATTCTCTG
AAATTGGAGCCATTGCAAAATCATTATTACCTTTAGTATCTTCAAGAAGTGATAACATCA
CAACCATCACCGTTAAGATAGACGCACACACTCTTTGTATGCTCTCACCCAT

Last Updated on 06.12.2018 16:11