Sequences from confirmation amplicon


Confirmation sequences for line 003C10 and hit At1g59890 are shown.
Line ID 003C10
Locus At1g59890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22049475
Primers used for PCR 01uv - 8474, show primer details
   
Sequence Name 57-K118051-0022-003-C10C-01uv
GenBank Accession LN509538 [GenBank]
Sequencing primer gene specific primer, 01uv: 5'-TTCCTGGTTTTCTGTAGTTCTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K118051-0022-003-C10C-01uv
AAGCATGTCCAAGCCACTTAAATGATTAGTGCGGAGCAAACAAAAATAAAGGAGAGAGAA
CAGAAGCAGAGTCAAGACGATAACAAGGTAAAATGGAAGCTTACATGCTTAACAAACTTT
TGAACTAGCTTGTCCAATGTGAAGAGAACATATGATTGTGCTCCGATAATAGCTCGACAT
TCATCTTCAAACTTTGTATTGTCGCTAGAGCCATCAAGTAAATTGTAAAGGGCTTCCATG
AACCTAAGCTTTCAAATATCAGTTAGGAAATAATATTTTTTCAATTAAAAAGTACCAATG
ATGCTTAGAAAATAAACCACCTGGTATAAGAAAAATACAAATACATACTAAGTATCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGGCTTTTTATTAAACAG
GGTGGTTTATTTTCTAAGCATCATTGGTACTTTTTAATTGAAAAAATATTATTTCCTAAC
TGATATTTGAAAGCTTAGGTTCATGGAAGCCCTTTACAATTTACTTGATGGCTCTAGCGA
CAATACAAAGTTTGAAGATGAATGTCGAGCTATTATCGGAGCACAATCATATGTTCTCTT
CACATTGGAACAGCTAGTTCAAAAGTTTGTTAAGCATGT
   
Sequence Name 21-K118050-0022-003-C10C-8409
GenBank Accession LN495850 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118050-0022-003-C10C-8409
TATTTTTCCTTATACCGGGTGGTTTATTTTTCTAAGCATCATTGGTACTTTTTAATTGAA
AAAATATTATTTCCTAACTGATATTTGAAAGCTTAGGTTCATGGAAGCCCTTTACAATTT
ACTTGATGGCTCTAGCGACAATACAAAGTTTGAAGATGAATGTCGAGCTATTATCGGAGC
ACAATCATATGTTCTCTTCACATTGGACAAGCTAGTTCAAAAGTTTGTTAAGCATGTAAG
CTTCCATTTTACCTTGTTATCGTCTTGACTCTGCTTCTGTTCTCTCTCCTTTATTTTTGT
TTGCTCCGCACTAATCATTTAAGTGGCTTGGACATGCTTCAGCTTCACGCAGTCGCAGCT
GATGAGACAGACACCAAGCTTCTGCAGCTATATGCATACGAGAACTACAGAAAAACCAGG
A

Last Updated on 06.12.2018 16:11