Sequences from confirmation amplicon


Confirmation sequences for line 003D03 and hit At5g19560 are shown.
Line ID 003D03
Locus At5g19560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6605737
Primers used for PCR MF23 - 8474, show primer details
   
Sequence Name 19-K031809-022-003-D03C-8409
GenBank Accession LN494647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K031809-022-003-D03C-8409
TTACAACAAACTCTTACTCAGTCTCACTACATTTTTGTTGATGTTTGTTTAGTCTTTGTT
ATGTTATGTTACCTTGTTGTTTTTAATCTTGGAGACTTCAAGGGAGGATTGTGGGATCCC
AGGAAATTGTTGTTTGAGCATAACCAAAATGGTCTCAGCTCGGACTTCGAAAAGCTCCCT
CTTCTCTAAGCTAACAGTGGATCCCCACTGAGATTTCCCGTCTTTCTCTTTCTGGCACAT
CTTCCTCTTCCAGATCACCATGGAAGCTTCAATCCTGTTCTTTAGATCAAGTACTTTGTG
TTCCGTTGACAAATCCAAGAAAGAGACAAAGTAGTCAGGATCAAAGCTCTCCTCTGTTAT
GCTCTTGTAAAGAAAATCTCCCAAGCTTGTCTTCCCATTCTACATAACCAACAGCCTCAT
CAGCTTCCTAGCTTAGTGGAAATTGTAAAGAAACAGAACACTTTTGAAGAAGAAGTAAGT
AACCTTTGGAAGGGAATCAATGTAGTTTTCAGGAACGTGCATTTCAGACAATACATGAGC
ATTGATGGCCGTGGCGGCTTTAAGGACTTGAGCCACAGAATCTTTCTGATTCTGCCACCA
TCT
   
Sequence Name 20-K031809-022-003-D03C-MF23
GenBank Accession LN495099 [GenBank]
Sequencing primer gene specific primer, MF23: 5'-AGATGGTTGCAGAATCAGAAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K031809-022-003-D03C-MF23
AGCGCCCGGCCATCAATGCTCATGTATTGTCTGAAATGCACGTTCCTGAAAACTACATTG
ATTCCCTTCCAAAGGTTACTTACTTCTTCTTCAAAAGTGTTCTGTTTCTTTACAATTTCC
ACTAAGCTAGGAAGCTGATGAGGCTGTTGGTTATGTAGAATGGGAAGACAAGCTTGGGAG
ATTTTCTTTACAAGAGCATAACAGAGGAGAGCTTTGATCCTGACTACTTTGTCTCTTTCT
TGGATTTGTCAACGGAACACAAAGTACTTGATCTAAAGAACAGGATTGAAGCTTCCATGG
TGATCTGGAAGAGGAAGATGTGCCAGAAAGAGAAAGACGGGAAATCTCAGTGGGGATCCA
CTGTTAGCTTAGAGAAGAGGGAGCTTTTCGAAGTCCGAGCTGAGACCATTTTGGTTATGC
TCAAACAACAATTTCCTGGGATCCCACAATCCTCCCTTGAAGTCTCCAAGATTAAAAACA
ACAAGGTAACATAACATAACAAAGACTAAACAAACATCAACAAAAATGTAGTGAGACTGA
GTAAGAGTTTGTTGTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATGAAACGACAAATTACTAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAGATCTTTATTTC
GACGTGTCTACATTC
Primers used for PCR FN26 - 3144, show primer details
   
Sequence Name 56-K032733-022-003-D03C-FN26
GenBank Accession LN509013 [GenBank]
Sequencing primer gene specific primer, FN26: 5'-TGTGTTTTTCTTCTTCTATGCCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K032733-022-003-D03C-FN26
GTCTGTCCTAGGGCTTCTCATGTTCTCTAACTTTGCTAGATATGAAAATTTCTTCGGTAG
AGCTCTTGGAGACTTCATCATGGGCTTCTCCTTCTCCTGCTCAACTTTGTGGAAGAATTC
TATATCACTCATTGAACTATGCTTCTTAGTATCAGATGACAATCTCCACCCTATGAAGTC
TGATAGTTTCCTCGAGTTTGGTGTTTCCGCTGCAACCACCTTTTCAGTTTCCTCGTATTC
TGATTCTGCCTCGCTTTCTGATCTTGTCTCTGCCTCTCCCATCAACTGTCTTTGAACTAG
CCGATCGGCTTCAAGAACATCCTCTATTCTTGACATTATCTTTGAAGCAAGACTCTCTAG
TACCCTTGAATAGCTCTCCAAAATCGCCAATCTGATAGTTTAAACCGAAGGCGGGAAACG
ACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGG
AAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
ATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCG
TGGGTGG
   
Sequence Name 24-K032733-022-003-D03C-3144
GenBank Accession LN496894 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K032733-022-003-D03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGATTGGCGA
TTTTGGAGAGCTATTCAAGGGTACTAGAGAGTCTTGCTTCAAAGATAATGTCAAGAATAG
AGGATGTTCTTGAAGCCGATCGGCTAGTTCAAAGACAGTTGATGGGAGAGGCAGAGACAA
GATCAGAAAGCGAGGCAGAATCAGAATACGAGGAAACTGAAAAGGTGGTTGCAGCGGAAA
CACCAAACTCGAGGAAACTATCAGACTTCATAGGGTGGAGATTGTCATCTGATACTAAGA
AGCATAGTTCAATGAGTGATATAGAATTCTTCCACAAAGTTGAGCAGGAGAAGGAGAAGC
CCATGATGAAGTCTCCAAGAGCTCTACCGAAGAAATTTTCATATCTAGCAAAGTTAGAGA
ACATGAGAAGCCCTAGTGACAGACACTGATAGTGAAAAGAGAAGGTAATAGAACAGGGCA
TAGAAAAGAAAAA

Last Updated on 06.12.2018 16:11