Sequences from confirmation amplicon


Confirmation sequences for line 004D10 and hit At4g36350 are shown.
Line ID 004D10
Locus At4g36350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17175798
Primers used for PCR 70mk - 8474, show primer details
   
Sequence Name 54-K114191-0022-004-D10C-70mk
GenBank Accession LN508551 [GenBank]
Sequencing primer gene specific primer, 70mk: 5'-CCAAATTTTGACTTTTAAGAAGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114191-0022-004-D10C-70mk
AAAGTTTGTTTCGAAATACGACCATGATAATCTCAATAACCGTTTAATATCTATAACTAG
GCTATAAGAATCCTATTTCTATTCGAATATTCTTTAAAATCCAACTTTATCTCTTCTTTT
GCAAAAAAAAAAAAACTTCAATTTAAGTTTCAAAAAAAAGGTTTGCCCCCCACCTTTTAA
CCCAAAAACCAAAAGAAACCTTCCCAAAATTTTTTAAGCCGGGGCCATTTAGGTTTAAAT
TTGGGGGGGAAAAAAATTTTCCTGGGTTTGGGGGTTCCAAACCTTGCCCCGGGAAACAAT
TGGAAAGGGGTTCCGGGCCGGGAAATTCCCCGGGGACCCCCAGGGGTTAGAGGGGTAAAT
GGGGAAAAAAAAAAGGGTTATTCCCCTTTTTTTTTCAATTCAAAAAGGGAAATTTCCCAA
ACGTTTTAAAAA
   
Sequence Name 19-K114190-0022-004-D10C-8409
GenBank Accession LN494853 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114190-0022-004-D10C-8409
ATTGATTACGTGGCAATGCTTAGAAACACAAAAACAAGTAAAATTTTGTTCATCCTCATA
TTTGATCTATGATCGTCGCTGCTTAAAAAGTTTTGAGTATGTTTCTTTCTGTTTTCTGAT
TAAACGTATGGGTGACAACGTTTTATATAGAACTTAAATTGAAGTTTTTTTTTTTTTTCA
AAAAAAAAAAAAAATTTGGATTTTAAAAAAATTTCCAAAAAAAAAAGGATTTTTAAACCC
CATTTTAAAAATTTAAACGGGTTTTGGAAATTACCGGGGCCTTTTTTCAAAAAAAACTTT
TTGGGAAAAGGTTGCAAAATTAACCCCCAAAAACAAAATTTTTTCCCTTCTAAAAATAAA
AAATTTGGG

Last Updated on 06.12.2018 16:11