Sequences from confirmation amplicon


Confirmation sequences for line 004D12 and hit At4g24050 are shown.
Line ID 004D12
Locus At4g24050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12499293
Primers used for PCR 02oz - 8474, show primer details
   
Sequence Name 31-K114963-0022-004-D12C-02oz
GenBank Accession LN500282 [GenBank]
Sequencing primer gene specific primer, 02oz: 5'-CTTCTTAGCTACATGGGTCGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K114963-0022-004-D12C-02oz
CCCATAATTTGGTAGCTTCGCTGGAGTTGGTTCCGAGTCCAGACGGTGTCGTTTCATTAC
AATCCGTAAAATACTTTCCCGATACGTTCACCAGTCTTGGATTTGTTGCCACGTAACACG
TCGTTGCTGCTGCCTAATCCCATGCCAAAACAAAAAAAAAGTAGCACATAAATCCAATAA
TCTTAAACAGGATTAAAAACAAAAAGAATAACTTATCCACCACAGATTATGTTACAAACT
TTATAACTAACGAAAATAACAATTATTTACTTGAGGGACGGTCTTAACGAGCTTGGAAGC
CAGGAAGAAGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAAATTTCCG
   
Sequence Name 15-K114962-0022-004-D12C-8409
GenBank Accession LN493092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114962-0022-004-D12C-8409
ATTTCATTGAATATATCCTGGCTTCTTCCTGGCTTCCAGCTCGTTAAGACCGTCCCTCAA
GTAAATAATTGTTATTTTCGTTAGTTATAAAGTTTGTAACATAATCTGTGGTGGATAAGT
TATTCTTTTTGTTTTTAATCCTGTTTAAGATTATTGGATTTATGTGCTACTTTTTTTTTG
TTTTGGCATGGGATTAGGCAGCAGCAACGACGTGTTACGTGGCAACAAATCCAAGACTGG
TGAACGTATCGGGAAAGTATTTTACGGATTGTAATGAAACGACACCGTCTGGACTCGGAA
CCAACTCCGCGAAGCTACCAAATTATGGGCTGCTTCTGAAGATCTGGTTACTCAACATTC
CAGACCAGTTTCGACCCAGGTAGCTAAAAA

Last Updated on 06.12.2018 16:11