Sequences from confirmation amplicon


Confirmation sequences for line 004E10 and hit At1g61500 are shown.
Line ID 004E10
Locus At1g61500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22690544
Primers used for PCR 22mm - 8474, show primer details
   
Sequence Name 22-K114225-0022-004-E10C-22mm
GenBank Accession LN496210 [GenBank]
Sequencing primer gene specific primer, 22mm: 5'-CTCTTCTCGATTCTCTGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114225-0022-004-E10C-22mm
GAAGCTGCAGATGGGAAAGAATTGCTGTTAAACGGCTTTCTAGCAGCTCTGGACAGGGAA
AAGAGGAGTTTATGAATGAAATAGTACTCATCTCAAAACTCCAACACAGAAACTTAGTCA
GGGTTTTGGGATGTTGCATCGAAGAAGAAGAGAAGCTATTGATTTATGAGTTTATGGTGA
ACAAAAGCCTTGATACCTTTCTCTTTGGTTGGTCCCTTCTTGCATCCTTCCTTTACTATT
CAGTAACTTTTTATATATATGTGGAAACTGTTTGTCTGACAGTTTTATTTCTTAGATTCA
AGAAAAAGGCTGGAGATTGATTGGCCAAAAAGATTTGATATCATTCAAGGTATTGCACGT
GGACTTCTCTATCTCCACCATGACTCACGCCTCAGAGTTATTCACCGAGACCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCC
   
Sequence Name 02-K114224-0022-004-E10C-8409
GenBank Accession LN487128 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114224-0022-004-E10C-8409
TGGTGGAGATAGAGAAGTCCACGTGCAATACCTTGAATGATATCAAATCTTTTTGGCCAA
TCAATCTCCANGCCTTTTTCTTGAATCTAAGAAATAAAACTGTCAGACAAACAGTTTCCA
CATATATATAAAAAGTTACTGAATAGTAAAGGAAGGATGCAAGAAGGGACCAACCAAAGA
GAAAGGTATCAAGGCTTTTGTTCACCATAAACTCATAAATCAATAGCTTCTCTTCTTCTT
CGATGCAACATCCCAAAACCCTGACTAAGTTTCTGTGTTGGAGTTTTGAGATGAGTACTA
TTTCATTCATAAACTCCTCTTTTCCCTGTCCAGAGCTGCTAGAAAGCCGTTTAACAGCAA
TTTCTTTCCCATCTTGCAGCTTTCCCTAAAACAGAATTGCAGAAGGTCATTTTGAGAGTA
TAAACAGAGAACNNAGAAGA

Last Updated on 06.12.2018 16:11