Sequences from confirmation amplicon


Confirmation sequences for line 005H01 and hit At5g01520 are shown.
Line ID 005H01
Locus At5g01520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:207617
Primers used for PCR 01vr - 8474, show primer details
   
Sequence Name 53-K118077-0022-005-H01C-01vr
GenBank Accession LN508291 [GenBank]
Sequencing primer gene specific primer, 01vr: 5'-GATGAATGGTGAGAGTGAGTGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K118077-0022-005-H01C-01vr
GCAGTTTATACACATGGAGTGATTGCACGTAGGAAGAACAACTTTGTTTCGAATCTCCAA
GCAGATTCCACACTCTTCTTCCCTCTCCAAATCGATCTCCGACATCTTTCCTTTATCTGT
TCTGTCCTTTTTACGGTATCTTTTGTCGCATATCTCCTTCTGTTTCCTTTCTTCTACATC
GGTGATCCCTCCATGAAGTTGCAATAGCGAAGGAAACAACACATCTGCCCAACAACATCC
ATTGCTAGTCAGAAAAAAACATATCGTCTGGTCTAATCTTATACCTTTAGATTAAGAGAG
AATAAGTGATTAACAGATTACCATAGAATTCTCTGATACTAGTTTTGCGTTCATGTAGCG
ACATTGTGGTCTTCCCATCAACATATGCCTATAAATAAAAAACAGAGAATCACATATCAG
GCAGACAAGAACATTGTGATCTTGAAGATCCAGTAGCAATAAAACAGCAGGAAACGTATG
TGTTACGCCAAGGCAAGACCATTACCTCAGTGTTATATATAGGTGTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 28-K118076-0022-005-H01C-8409
GenBank Accession LN499034 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118076-0022-005-H01C-8409
TTACATTGAACACCTATATATAACACTGAGGTAATGGTCTTGCCTTGGCGTAACACATAC
GTTTCCTGCTGTTTTATTGCTACTGGATCTTCAAGATCACAATGTTCTTGTCTGCCTGAT
ATGTGATTCTCTGTTTTTTATTTATAGGCATATGTTGATGGGAAGACCACAATGTCGCTA
CATGAACGCAAAACTAGTATCAGAGAATTCTATGGTAATCTGTTAATCACTTATTCTCTC
TTAATCTAAAGGTATAAGATTAGACCAGACGATATGTTTTTTTCTGACTAGCAATGGATG
TTGTTGGGCAGATGTGTTGTTTCCTTCGCTATTGCAACTTCATGGAGGGATCACCGATGT
AGAAGAAAGGAAACAGAAGGAGATATGCGACAAAAGATACCGTAAAAAGGACAGAACAGA
TAAAGGAAAGATGTCGGAGATCGATTTGGAGAGGGAAGAAGAGTGTGGAATCTGCTTGGA
GATTCGAAACAAAGTTGTTCTTCCTACGTGCAATCACTCCATGTGTATAAACTGCTACAG
AAACTGGTATATAAAGCTTTTCCTTCTTTTCACTCACTC

Last Updated on 06.12.2018 16:11