Sequences from confirmation amplicon


Confirmation sequences for line 005H06 and hit At1g65486 are shown.
Line ID 005H06
Locus At1g65486 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24349614
Primers used for PCR 06mk - 8474, show primer details
   
Sequence Name 24-K114225-0022-005-H06C-06mk
GenBank Accession LN497087 [GenBank]
Sequencing primer gene specific primer, 06mk: 5'-ACACTGAAGATAGGGTGAACGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114225-0022-005-H06C-06mk
CCATATTAATAGTCACTCATATTGAATATTCCTCTCTGCTAATATTAAATGGCAACGAAG
TCGTCTTTGATTTTTTTTTTTTCCCCAAAAAAAGGGACAAAACACTTATCTTTAAAATTC
AAATATTTCTAGCCTTTCGTTTATCTAACCTAATAGGCTTGAACCTAATGTAAAACCCGC
GTTCACTAGTGAAAATTTGCAAATAAGGTTAAATACCAAAAGCAAACAAATTTGACTTTC
CCCTCCTTTTATATATCTGGCATTTGCCATTTCCAATAACCAAAAACTTGACAAAAAAAA
AAAGGGGTTTGATGTCCCCAAATATCGCTCCATTTGTGTTACCAAGGCTCCTGGTACTTT
TCACAATTTCCCCTCAGGTTAAATTTGGGGAATCATATTCAATTGTAAAGGGCTCCATGT
CCGGGAAATCTACTGGGATCACCATTGAGTATAAGGGTCAATATGGAAAAAAAAAAAAAG
TATTTCCCATTTTTTTTTCATTTCAAAAATGTAAAT
   
Sequence Name 04-K114224-0022-005-H06C-8409
GenBank Accession LN488007 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114224-0022-005-H06C-8409
ATTACATTGAATATGATTCCACAACTTCAACTTGAGAGGAAATAGTGAAAAGTACAAGAA
GCATTGGTAACACAAATGCAGCGATATTTGGTGACATCAAACCCATTTTTTTCTTCGTCA
TGTTTTTGGTCATCGGAATTGGCAAGTGCTAGATATATAAAAGAAGCGTAACGTCATATT
TGTTTGCTTTTGCTATTTAACTTTATTTGCAAATTTTCACTAGTGAACGCGGTTTCTACA
TCAGCTACAAGCCTATAAGATTAGATAAACGAATGGCTAGAAATATTTGAATTTAATAGA
TAAGTGTTTTGTCCCTTCTTTTGGGGAAAAAAAAAAAATCAAAAACAACTTCTTTGCCTT
TTAATATTAGCAAAGAGGAATATTCAATATGATTTGACTAATTAATATGGGCCGTTGACT
TAGGTGTATAAAAACAACTCGTTCACCC

Last Updated on 06.12.2018 16:11