Sequences from confirmation amplicon


Confirmation sequences for line 006E12 and hit At3g61510 are shown.
Line ID 006E12
Locus At3g61510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22764210
Primers used for PCR AT22 - 8474, show primer details
   
Sequence Name 05-K025391-022-006-E12C-8409
GenBank Accession LN488198 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K025391-022-006-E12C-8409
TTGAAATATATCCTAATTCAGAGATCATCCAAGAGATGTATTATGTTAACCGTGATCTGA
TTCATATCGTCTACAGTCTTTCAAAGGACATGGGTCTTCCCGGTTTCCGGGTTGGAGTGG
TTTACTCTTACAACGATGTTGTTGTGTCCTGCGCAAGGAGGATGTCGAGTTTTGGATTGG
TCTCGTCGCAGACACAAAGTTTTCTAGCTGCTATGTTGTCTGATCAGAGTTTTGTCGATA
ACTTTCTTGTTGAGGTTTCGAAAAGAGTAGCGAAGAGACACCATATGTTCACGGAAGGGC
TTGAAGAGATGGGGATTTCTTGCTTGAGAAGCAACGCGGGTTTATTCGTTTTGATGGATT
TGAGGCATATGCTTAAGGATCAGACATTTGATTCCGAAATGGCGCTTTGGCGAGTTATTA
TCAATAAGGTCAAGATTAATGT
   
Sequence Name 06-K025391-022-006-E12C-AT22
GenBank Accession LN488648 [GenBank]
Sequencing primer gene specific primer, AT22: 5'-GCCAGGAGAGACATTAATCTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K025391-022-006-E12C-AT22
CTCGCCAAAGCGCCATTTCGGAATCAAATGTCTGATCCTTAAGCATATGCCTCAAATCCA
TCAAAACGAATAAACCCGCGTTGCTTCTCAAGCAAGAAATCCCCATCTCTTCAAGCCCTT
CCGTGAACATATGGTGTCTCTTCGCTACTCTTTTCGAAACCTCAACAAGAAAGTTATCGA
CAAAACTCTGATCAGACAACATAGCAGCTAGAAAACTTTGTGTCTGCGACGAGACCAATC
CAAAACTCGACATCCTCCTTGCGCAGGACACAACAACATCGTTGTAAGAGTAAACCACTC
CAACCCGGAAACCGGGAAGACCCATGTCCTTTGAAAGACTGTAGACGATATGAATCAGAT
CACGGTTAACATAATACATCTCTTGGATGATCTCTGAATTAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGC
GAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11