Sequences from confirmation amplicon


Confirmation sequences for line 006F04 and hit At4g27350 are shown.
Line ID 006F04
Locus At4g27350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13692569
Primers used for PCR 71mk - 8474, show primer details
   
Sequence Name 48-K114191-0022-006-F04C-71mk
GenBank Accession LN506569 [GenBank]
Sequencing primer gene specific primer, 71mk: 5'-CCAGAAGACACTCAAACAAAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K114191-0022-006-F04C-71mk
CAGAGCTGAATCTCCATTAAATCTAATTCCTTCACATCAAGATCATCACAATCACCACAA
CAATCGAATAGTTTACAATGTTACGAAAACTCACCCGGAAGGCTGGAGAAGGAAACCTAG
GCGCTTCGGAGATAGATTTGAGCAGCGTCTCCTCTTCGTTTCCGATCAATTGAGAACGAC
CTTGGATCACAAGCTGCGGAGTAAACATCGTATCCAGATTCAACGCCTCTATATACGCCT
TCTGCCTCACCGTCCATTGGCTCGATCCATACGGATCTTTCCACCCCTTGTAATCCCAAT
AATCCACATGAAACACCAAAACCATCGTCGGAGATCCAGATCCACCATCTTCGCCACGGA
TCTGAGCGTCGAAATCACCTCGACCTAGCCGGGACATCAACATCTCCGCCGCGGGCGATG
TTTTACATCCCTGAGACGAGAATAGCTGGACGAGAACGGGACCATCTCCGGCGGGAATTT
CTGCGGCTACGGCGGCGGAATTGTTACCACCAGAGATAGATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 13-K114190-0022-006-F04C-8409
GenBank Accession LN492132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K114190-0022-006-F04C-8409
CATTTCATTGATCTATCTCTGGTGGTAACAATTCCGCCGCCGTAGCCGCAGAAATTCCCG
CCGGAGATGGTCCCGTTCTCGTCCAGCTATTCTCGTCTCAGGGATGTAAAACATCGCCCG
CGGCGGAGATGTTGATGTCCCGGCTAGGTCGAGGTGATTTCGACGCTCAGATCCGTGGCG
AAGATGGTGGATCTGGATCTCCGACGATGGTTTTGGTGTTTCATGTGGATTATTGGGATT
ACAAGGGGTGGAAAGATCCGTATGGATCGAGCCAATGGACGGTGAGGCAGAAGGCGTATA
TAGAGGCGTTGAATCTGGATACGATGTTTACTCCGCAGCTTGTGATCCAAGGTCGTTCTC
AATTGATCGGAAACGAAGAGGAGACGCTGCTCAAATCTATCTCCGAAGCGCCTAGGTTTC
CTTCTCCAGCCTTCCGGGTGAGTTTTCGTAACATTGTAAACTATTCGATTGTTGTGGTGA
TTGTGATGATCTTGATGTGAAGGAATTAGATTTAATGGTAGATTTCAGCTCTTGTTTATG
TGATTTCAATGTAAGTGTCTTGTTTTGTTTGAGNTCCTTCTGG

Last Updated on 06.12.2018 16:11