Sequences from confirmation amplicon


Confirmation sequences for line 007C11 and hit At1g06750 are shown.
Line ID 007C11
Locus At1g06750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2075158
Primers used for PCR 12qb - 8474, show primer details
   
Sequence Name 50-K115714-0022-007-C11C-12qb
GenBank Accession LN507277 [GenBank]
Sequencing primer gene specific primer, 12qb: 5'-GATTTATTTTGTATTAGAAAGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K115714-0022-007-C11C-12qb
TTTTGTGTGTAATGTATCTAGGGAGCTGAGGATTAAGAGGGTGACTAAGAACTTAAAAGT
GGCAAGAGTGTTCAACACTTTGGTAGAGGAAATGAGAGCAATGGGGCTCGCGTCTGTGGA
TGACTCGCAGTGCACAGAAGTTATGGCACCAGTGGCTCACAAAGACAGAAGTCCAGTGCT
ACTCCTTATGGGTGGTGGGATGGGTGCAGGGAAAAGCACTGTGCTTAAGGACATACTCAA
AGAGTAAATAAAGATCTCATTATATAATTATCACTTCTACTATACATATAGAGAAACGTA
TATATCTATGTAGCTGAAGCAAAGAAGCTAACCAAATGGTTTTCACTATGGAAGCAGAGC
ATTCTGGGCAGGAGCAGACTCTGTGGTGATTGAAGCTGATGCTTTCAAAGAGTCTGATGT
CATTTACAGAGCCTTAAGCTCTCGTGGCCATGTCGATATGATCCACAAACATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
   
Sequence Name 10-K115713-0022-007-C11C-8409
GenBank Accession LN490798 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115713-0022-007-C11C-8409
TATCGACATGGCCACGAGAAGCTTAGGCTCTGTAAATGACATCAGACTCTTTGAAAGCAT
CAGCTTCAATCACCACAGAGTCTGCTCCTGCCCAGAATGCTCTGCTTCCATAGTGAAAAC
CATTTGGTTAGCTTCTTTGCTTCAGCTACATAGATATATACGTTTCTCTATATGTATAGT
AGAAGTGATAATTATATAATGAGATCTTTATTTACTCTTTGAGTATGTCCTTAAGCACAG
TGCTTTTCCCTGCACCCATCCCACCACCCATAAGGAGTAGCACTGGACTTCTGTCTTTGT
GAGCCACTGGTGCCATAACTTCTGTGCACTGCGAGTCATCCACAGACGCGAGCCCCATTG
CTCTCATTTCCTCTACCAAAGTGTTGAACACTCTTGCCACTTTTAAGTTCTTAGTCACCC
TCTTAATCCTCAGCTCCCTAGATACATATACACACAAAATCAATTATTCGTCAATCACTA
AAACAGAACACACGGCTTTCTAATACANAATAAAT

Last Updated on 06.12.2018 16:11