Sequences from confirmation amplicon


Confirmation sequences for line 007F02 and hit At4g34050 are shown.
Line ID 007F02
Locus At4g34050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16311649
Primers used for PCR LJ07 - 8474, show primer details
   
Sequence Name 09-K027486-022-007-F02C-8409
GenBank Accession LN490032 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K027486-022-007-F02C-8409
GCCATTCACAATTGAAAAGGAAAAAATTTGCGTATACAGAAATTCTCATCAAGATATTAA
ATCAACCTAATTAGGCCATGAGTTTTTAATTATTTCATTATCTTCATCATCTTTTTTGTT
AATTACGTCAGAAAGAAGAATGCTTACGTCAGCAACGATTTCATCAAGAACGGGAAGAGC
AGGGCCTTCCCTGAAGTCGATCTTGTGAGCAACGCCGGCTTTCTCAATGATCGGTAAACC
CAATTCGTAATTCTCTCTGTTGACATCCATAGCCAGAATCTGATGATAAAAAGGATGAAA
CTTTCAATTCCCAAGTTCGACAAAACCCACCAAAAAACAGAGGAGGATGAAGAGATTGAT
TTACTTTGCCGTCTTCAGGGAGAGCAAGAGCGGTGGCGAGAAGAGAGTAGCCAGTGTAAA
CTCCGATCTCCATTGTGTTCTTGGCGTTAACGAGCTTGATAAGCATGTTTAAGAACTGTC
CTTCATCAGCTGATGTGGTCATTATGTTCCATGGATGTTTTGCTGTCACTTCCCTGAGTT
CCT
   
Sequence Name 10-K027486-022-007-F02C-LJ07
GenBank Accession LN490506 [GenBank]
Sequencing primer gene specific primer, LJ07: 5'-AGAGAACCAGAATCAATGAAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K027486-022-007-F02C-LJ07
ACATCCAGGAACAAATGACCACATCAGCTGATGAAGAAGTTCTTAAACATGCTTATCAAG
CTCGTTAACGCCAAGAACACAATGGAGATCGGAGTTTACACTGGCTACTCTCTTCTCGCC
ACCGCTCTTGCTCTCCCTGAAGACGGCAAAGTAAATCAATCTCTTCATCCTCCTCTGTTT
TTTGGTGGGTTTTGTCGAACTTGGGAATTGAAAGTTTCATCCTTTTTATCATCAGATTCT
GGCTATGGATGTCAACAGAGAGAATTACGAATTGGGTTTACCGATCATTGAGAAAGCCGG
CGTTGCTCACAAGATCGACTTCAGGGAAGGCCCTGCTCTTCCCGTTCTTGATGAAATCGT
TGCTGACGTAAGCATTCTTCTTTCTGACGTAATTAACAAAAAAGATGATGAAGATAATGA
AATAATTAAAAACTCATGGCCTAATTAGGTTGATTTAATATCTTGATGAGAATTTCTGTA
TACGCAAATTTGTTTCCTTTTTCAATTGTGAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAA
ACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC
GGAAATCTTT
Primers used for PCR CT30 - 8474, show primer details
   
Sequence Name 32-K118466-0022-007-F02C-CT30
GenBank Accession LN500765 [GenBank]
Sequencing primer gene specific primer, CT30: 5'-GACTTACGTTGCTGTGACACAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K118466-0022-007-F02C-CT30
ACGAGACCAGAAGAAAAGTAAGGTACATATTATTTCTTTGTTCAGAAGAAAGCAAAACAA
GGACAATGGAGATATTACTTAACACAAATACATAAAAAGGTCCATTTTAGTGGTTGGTGA
AAATGCGATTGAAAGGTATAAGAAGATGGAAAGTAATCCACTGTGGACAAACTCAGAGTA
GGGAGGAGTCAAATCAACTGATCCGACGGCAGATAGTGATTCCATCACCAACAGGGAGCA
TACAGATCTCGATCCGAGGGTCAGCAGCAAGAGCCTTGTTAAGCTCAAGAACAAAGTCTC
TGTAGTAACGAACGTACTTCCTCATTGGTGCATCAGGAGGAGCCACGACAGAACCATTCC
ACAGAGTGTTGTCGTAGCCAATCACTCCTCCAATTTTCACAAGATCGATCAAACGCTTGT
GGTAGTTGATGTAGTTGTCTTTGTCAGCATCAACGAATATAAAGTCATATGTTCCATGGT
TCTTCTCCTGCACCCAACCACACACAATAATCAGTTACAATAATCAGGATATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 05-K118465-0022-007-F02C-8409
GenBank Accession LN488557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118465-0022-007-F02C-8409
ATTGAATATATCCTGATTATTGTAACTGATTATTGTGTGTGGTTGGGTGCAGGAGAAGAA
CCATGGAACATATGACTTTATATTCGTTGATGCTGACAAAGACAACTACATCAACTACCA
CAAGCGTTTGATCGATCTTGTGAAAATTGGAGGAGTGATTGGCTACGACAACACTCTGTG
GAATGGTTCTGTCGTGGCTCCTCCTGATGCACCAATGAGGAAGTACGTTCGTTACTACAG
AGACTTTGTTCTTGAGCTTAACAAGGCTCTTGCTGCTGACCCTCGGATCGAGATCTGTAT
GCTCCCTGTTGGTGATGGAATCACTATCTGCCGTCGGATCAGTTGATTTGACTCCTCCCT
ACTCTGAGTTTGTCCACAGTGGATTACTTTCCATCTTCTTATACCTTTCAATCGCATTTT
CACCAACCACTAAAATGGACCTTTTTATGTATTTGTGTTAAGTAATATCTCCATTGTCCT
TGTTTTGCTTTCTTCTGAACAAAGAAATAATATGTACCTTACTTTTCTTCTTGGTCTCGT
TCTTTTGTTTTTCTCCATGATACAACATCTAAAGAAATTATTTGTGTCA

Last Updated on 06.12.2018 16:11