Genotyping and primer details


Genotyping and primer details for 007F02 - At4g34050
Line ID 007F02
Locus At4g34050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16311649 of line 007F02 Chr4:16311649
   
Insertion allele amplicon 8474 - CT30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CT30: 5'-GACTTACGTTGCTGTGACACAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16312230
   
Insertion allele amplicon 8474 - LJ07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer LJ07: 5'-AGAGAACCAGAATCAATGAAGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16311083
   
wt amplicon (over InsPos) CT30 - LJ07
wt amplicon start Chr4:16311083
wt amplicon end Chr4:16312230
wt amplicon size 1148 bp
Locus specific primer LJ07: 5'-AGAGAACCAGAATCAATGAAGGAA-3'
2nd locus specific primer CT30: 5'-GACTTACGTTGCTGTGACACAAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11