Sequences from confirmation amplicon


Confirmation sequences for line 007H08 and hit At4g01430 are shown.
Line ID 007H08
Locus At4g01430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:586616
Primers used for PCR BC30 - 8474, show primer details
   
Sequence Name 01-K104268-0022-007-H08C-BC30
GenBank Accession LN486557 [GenBank]
Sequencing primer gene specific primer, BC30: 5'-AAAAACTTACAGCGTAAAGGGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104268-0022-007-H08C-BC30
ATCCATCTTTGACGTCTCTACTTTTATAAAGACTCAAAATAGCACATTGACATGATGCGA
AGACTGACATGAGACAAGTGCTTGAGTATTTATTTCCAGGATACTTAAAGCTTAATTTCC
CTTGAAACAACATCCATAGAGACAACAACACTGTCCCAATGACTAAATAAAGACAGCCTA
GAAGCCATTTCTTTGTTTGGTCGTGACCATTGTTATTGTTGTTGTTGTGACGAGCTTGTG
GATGAGAATGAGGGTTTGATAACTCTGGGCCTTTGTAAAATGTCAACAACATTGCTCCCA
TTATGCAAATTAGGGTTCCCATTACTTTGAGCACGCCTGCTTTGCTCTTTAGGTTTTGCA
TCATGTAAATTAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 17-K104269-0022-007-H08C-8409
GenBank Accession LN493833 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104269-0022-007-H08C-8409
AAGCAGGCGTGCTCAAAGTAATGGGAACCCTAATTTGCATAATGGGAGCAATGTTGTTGA
CATTTTACAAAGGCCCAGAGTTATCAAACCCTCATTCTCATCCACAAGCTCGTCACAACA
ACAACAATAACAATGGTCACGACCAAACAAAGAAATGGCTTCTAGGCTGTCTTTATTTAG
TCATTGGGACAGTGTTGTTGTCTCTATGGATGTTGTTTCAAGGGAAATTAAGCTTTAAGT
ATCCTGGAAATAAATACTCAAGCACTTGTCTCATGTCAGTCTTCGCATCATTTCAATGTG
CTATTTTGAGTCTTTATAAAAGTAGAGACGTCAAAGATTGGATCATTGAAGATAAATTCG
TCATTTTGGTCACCCTTTACGCTGTAAGTTTT

Last Updated on 06.12.2018 16:11