Sequences from confirmation amplicon


Confirmation sequences for line 008F05 and hit At3g46120 are shown.
Line ID 008F05
Locus At3g46120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:16938819
Primers used for PCR 09mk - 8474, show primer details
   
Sequence Name 29-K114405-0022-008-F05C-09mk
GenBank Accession LN499396 [GenBank]
Sequencing primer gene specific primer, 09mk: 5'-GAACAAAGAAAAGTTAAAGAAAAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K114405-0022-008-F05C-09mk
AGAGATTTTAGTACATTATAATTAATTGAATGTAGTTCAATAAAGATAAAATTTAATTAA
TGATATAAATTTAGACATATATATATATTTTTTGATACTAAATATATAATAAAGGAAAGA
GTTAATTCATGTATTTAATGTCAACTAAGGAATTGTAAGTTATTAGATATTTGGTTGAGA
CATAATTTGTAAAGACTTAAATTTAATAGTAAATAATATATGTAGAAGTTTAAGACAAGG
TCTTTAATGATTTAGTAACTTTTACTAAACTTTTAAAAACCCTTCTGAACTTACGTTTGT
GGTATATTTGTAACTTCTTACAAGGTAATTATTTGGGTGATAATTAGTAATTTAGTAGAA
TTGCTTTACAGACGAAAGTAAACTTCATCATTTGTTTTTTTTCCTTGTTTTCTAGCATTG
ATTTTTACTTAACCTGTATAATGAGAAGTTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 07-K114404-0022-008-F05C-8409
GenBank Accession LN489372 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K114404-0022-008-F05C-8409
ATTTACACTTCTCATTATACAGGTTAAGTAAAAATCAATGCTAGAAAACAAGGAAAAAAA
ACAAATGATGAAGTTTACTTTCGTCTGTAAAGCAATTCTACTAAATTACTAATTATCACC
CAAATAATTACCTTGTAAGAAGTTACAAATATACCACAAACGTAAGTTCAGAAGGGTTTT
TAAAAGTTTAGTAAAAGTTACTAAATCATTAAAGACCTTGTCTTAAACTTCTACATATAT
TATTTACTATTAAATTTAAGTCTTTACAAATTATGTCTCAACCAAATATCTAATAACTTA
CAATTCCTTAGTTGACATTAAATACATGAATTAACTCTTTCCTTTATTATATATTTAGTA
TCAAAAAATATATATATATGTCTAAATTTATATCATTAATTAAATTTTATCTTTATTGAA
CTACATTCAATTAATTATTAATTTACTAAAATCTCTCTATATACATTAAATATAAATATA
TGATTTTTCTTTAACTTTTCTTTGTT

Last Updated on 06.12.2018 16:11