Sequences from confirmation amplicon


Confirmation sequences for line 008H09 and hit At5g52170 are shown.
Line ID 008H09
Locus At5g52170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21199704
Primers used for PCR 09ne - 8474, show primer details
   
Sequence Name 35-K114376-0022-008-H09C-09ne
GenBank Accession LN501925 [GenBank]
Sequencing primer gene specific primer, 09ne: 5'-GCAAATTGTAAACTTTGGTCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K114376-0022-008-H09C-09ne
TCTCAATTTGGCTACCTCTCACAAATTGTTTCAGACATAGAAACAAGATTAGGTCCTACA
ACTTTAGCTAAGCAGGTATACGAAGAGCGGCTCTGATCTTGTGAATGGTGCAAGCGATGA
GGTTGTTAACAGTTTCAACAGATTCCACGTTGAGTTTTGCAGTCGGTAGGCTGTTCACTA
AGATCTGAAATCCTACAGTCAGGAGACATCCTTTGCTTTCTTGATTCACCAAACCGCCAT
CGGTATCGAACTGATCAGACGATGATGATGATCCGTCAGGCAATATCGAGAACCCCGATG
GAAGAAGCGCCACATAAGCTGAAAAGAAGCGACAATCTGATCCCCGGGTACGAAGCACTG
ATAGTTTAAACCGAGTCCTTGTGTCCTGGGAAATCTACATGGATCAGCATGGATCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCA
   
Sequence Name 21-K114375-0022-008-H09C-8409
GenBank Accession LN495762 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K114375-0022-008-H09C-8409
ATCCTGATCCATGCTGATCCATGTAGATTTCCCAGGACACAAGGACTCGGTTTAAACTAT
CAGTGCTTCGTACCCGGGGATCAGATTGTCGCTTCTTTTCAGCTTATGTGGCGCTTCTTC
CATCGGGGTTCTCGATATTGCCTGACGGATCATCATCATCGTCTGATCAGTTCGATACCG
ATGGCGGTTTGGTGAATCAAGAAAGCAAAGGATGTCTCCTGACTGTAGGATTTCAGATCT
TAGTGAACAGCCTACCGACTGCAAAACTCAACGTGGAATCTGTTGAAACTGTTAACAACC
TCATCGCTTGCACCATTCACAAGATCAGAGCCGCTCTTCGTATACCTGCTTAGCTAAAGT
TGTAGGACCTAATCTTGTTTCTATGTCTGAAACAATTTGTGAGAGTGTAGCCNAATTTGA
GACTGAGTAGCTNGTAGAACAAACAAATAAACAAACAAATGGACCAAAGTTA

Last Updated on 06.12.2018 16:11