Sequences from confirmation amplicon


Confirmation sequences for line 009A05 and hit At4g15210 are shown.
Line ID 009A05
Locus At4g15210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8668477
Primers used for PCR 11co - 8474, show primer details
   
Sequence Name 26-K108475-0022-009-A05C-11co
GenBank Accession LN497963 [GenBank]
Sequencing primer gene specific primer, 11co: 5'-CATCCTTCTTGAAAAATCCAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K108475-0022-009-A05C-11co
TCGGCGTCCTCGGCAAGTCCACTCAGGGTGCCCTGCTTTCGCCGCCGCTTCCTTGAAATC
TTTCTTCAAGTACTTGTCATAACACTAAAATCACCAAAACGCACCNGTTTAGTGAGGGAC
AAAAATCATAAAAACAAAAGAAGTGTAACTAAATTTACAAAACTAACCTGGAATTCTCCG
ATGCCGGGAAACACCCAACCTTGGCTTTGGGGGTAAGAAGGATAACGTAGTTCACCGGCC
GGGCCAAGTCCGACTTCGATGTCAACAATCACCCCAGCTTCTATCAAATCCGCCATGTTT
TCTTTGAAGCTACTCATGTAATCACTGTACAACTAATGGAAAAAACAAAAGTAGTAATTT
GTTTATTTCAACTCTTTTTTTTTTTGGCCAAAAACATGCAATATGGGTACTTTTCTTGAA
GTTTACAACCGAAACAGGGGTTCTTCCGGCAAATGGGGAAAAATTTTCAACCCCGATGGA
AAAATACCCAATGTCTCTAGTTCCTTTACGGTAAGGGAAGAAAATATCGGGATCATTGTC
CCCAACTTCGCGGGAAATCTACTTGGATCACCAATGAGTATGAGGGCCAATTTGGAAAAA
AAAAAAAAGTAATTCCCATTTTTTTTTCATTTCAAAAATGAAAATGTCCCCACCTTTATT
   
Sequence Name 10-K108474-0022-009-A05C-8409
GenBank Accession LN490693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K108474-0022-009-A05C-8409
TGATCCCGATATCTACTACACTAACCGTAAAGGAACTAGAGACATCGAGTATCTCTCAAT
CGGTGTTGATAATCTTCCCCTATTTGCCGGAAGAACCGCTGTTCAGGTAGTAAACTTCAA
GAAAAGTACACATATTGCATGTTTTTGACCAAAAAAAAAAAAAGTTAAAATAAACAATTT
CCTCCTTTTGTTTTTCCCTTTAGTTGAACAGGGATTACTTGAGTACCTTCAAAAAAAACT
TGGGGAATTTAATAAAACCGGGGGGGATTGTTAACATCAAAGTCGAACTTGGCCCGGCCG
GGAAATTACTTTATCCTTCTTACCCCCAAAGCCAAGGTGGGGGGTTTCCCGGCTTCGAAA
AATTCCGGGTAAGTTTTGAAAATTAAGTTACCCTTCTTTGGTTTTTAGAATTTTGGCCCC
CCACAAAACGGGGCGTTTGGGGAATTTTAGGGTTATGACAAGTACTTGAAAAAAAATTTC
AGGGAACCGGCGGCAAAACCAGGGCACCCTGAGGGGAATTTGCCAAAGGACCCCGAAAAA
TACAATGACAAGCCGAAGGAAACGGGATTTTTCAAAAAGGAG
Primers used for PCR 05wr - 3144, show primer details
   
Sequence Name 84-K118629-0022-009-A05C-05wr
GenBank Accession LN514478 [GenBank]
Sequencing primer gene specific primer, 05wr: 5'-ATCTTGGAGGAAATTTGTATGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118629-0022-009-A05C-05wr
AGGATAGTGACTATACTAGATCTTGCCAAAAATATTTCCTATCATTTAGTTATGACTTTC
AGTCCGAGACTTGAATTTACGGAGACAAGGGAACATATAAATCTAATTCTTTGAATGACA
TAGCCACCATCATATGTGATTTAGCAATTACATAGTTTGTAATTCTATATATCTTTTCAC
ACGCTAAATAATGTGCATTTTCGCATTTATGCATATATAATGGTTCAAAATGTTTTTTTG
GTTTTTTGTTCTTTCTCAACAATGTCTTTGATCATAACTATCAATGAAAATATGTTGTGA
ATGTGACAGTTGGGAGTTGTGAATGTGGAAAATGTATTTGCGGACCCAGAAACGCTTGAA
ACGCAGCTTAAACGTCTCAAAGAAGAAGCTGGCGTTGATGGCGTTATGGTCGATGTTTGG
TGGGGAATCATAGAATCCAAAGGTCCCAAACAATATGATTGGACGGCCTACAAAACGCTG
TTCCAGCTGATCGCACGTTTGGGACTCAAAATCCAAGCAATCATGTCTTTTCACCAATGT
GGTGGAAACGTAACATTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGG
GTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGG
ATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
   
Sequence Name 65-K118628-0022-009-A05C-3144
GenBank Accession LN511498 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118628-0022-009-A05C-3144
CCTTCGCAGACCCTTCCTCTAATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAATGTTACGTTTCCACCAC
ATTGGTGAAAAGACATGATTGCTTGGATTTTGAGTCCCAAACGTGCGATCAGCTGGAACA
GCGTTTTGTAGGCCGTCCAATCATATTGTTTGGGACCTTTGGATTCTATGATTCCCCACC
AAACATCGACCATAACGCCATCAACGCCAGCTTCTTCTTTGAGACGTTTAAGCTGCGTTT
CAAGCGTTTCTGGGTCCGCAAATACATTTTCCACATTCACAACTCCCAACTGTCACATTC
ACAACATATTTTCATTGATAGTTATGATCAAAGACATTGTTGAGAAAGAACAAAAAACCA
AAAAAACATTTTGAACCATTATATATGCATAAATGCGAAAATGCACATTATTTAGCGTGT
GAAAAGATATATAGAATTACAAACTATGTAATTGCTAAATCACATATGATGGTGGCTATG
TCATTCAAAGAATTAGATTTATATGTTCCCTTGTCTCCGTAAATTCAAGTCTCGGACTGA
AAGTCATAACTAAATGATAGGAAATATTTTTGGCAAGATCTAGTATAGTCACTTATCCTT
TGTACGTTTATAATCAAAGAGTTCTGGCCGAAGCACATACAAATT

Last Updated on 06.12.2018 16:11