Sequences from confirmation amplicon


Confirmation sequences for line 009C02 and hit At1g10200 are shown.
Line ID 009C02
Locus At1g10200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3346992
Primers used for PCR ML03 - 8474, show primer details
   
Sequence Name 05-K030752-022-009-C02C-8409
GenBank Accession LN488232 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K030752-022-009-C02C-8409
GGAAGATAACCCTTTAATATATAAGGACATGTAGATTTCCCGGACATGAAGCCATTGCTG
ATCAGGAAAGATTTGAGATTGGATACCTTCTCAATTGGATACACGNGTTTTGTCGCAACC
AACGCATTTCTCTCGTGTTCCACCAAACATATTCGAAACTTTGGTTCCAGCAGGTCTCTG
TTAAACTTCTCTCATGTTAAATGTTAAAAAAATTCAAGAACCTCAAAAACAGAAAAAGGG
TTTTGTTCTGCTGTCCGAAAGTACCTCTCCCTCCAAAGGCCTATCAGGTTTCCCAATCTT
TGGTGTCCCTGTACATATATATGAATCAAACTTTAGATAGATAATTCCAAATTTGAAATC
CCCACTCACAAAGCTTTTCACATTTTGATCTGTTTTTGGTAATTTACCTTCGAAGCTTTT
CTCAAGACTTCCAGTTCTCTTGAAGTTTTGATCGAAATGTGAAAAGCTTTGTGAGTGGGG
ATTTCAAATTTGGAATTATNTATNNAAAGTTNGA
   
Sequence Name 44-K030752-022-009-C02C-ML03
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, ML03: 5'-CACATTTCGATCAAAACTTCAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K030752-022-009-C02C-ML03
AAAACTTATCCCAATGTGAAAAGCTTTGTGAG

Last Updated on 06.12.2018 16:11