Sequences from confirmation amplicon


Confirmation sequences for line 009C11 and hit At3g28360 are shown.
Line ID 009C11
Locus At3g28360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10612091
Primers used for PCR 42yx - 8474, show primer details
   
Sequence Name 12-K123449-0022-009-C11C-42yx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 42yx: 5'-GGAAAGATTTATTTACTCGGTCCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K123449-0022-009-C11C-42yx
TCTCGGATCTAAGAACACTTGTCTAGAAACTTAACCAAACGTGCAATGATGAAGGAAATA
ATACTAGTCCGTAGCTAGTTTCATCAACAGTGATCATGTTCTTTGGTTTTAAAAGGTATA
GACGCAATGAACCCCAGCATTATTTTTACACCAACAACTAACAAACCCAATAATACGTCT
ACATGGTAACATGCTTCATGTTTTAGGCTTGTGAAACATATGTGAATTTATAATTTATAC
ATATCTGACCTTTCTCTGAAGACTGACCAATGAGAAATAAGAGCCTGTGGGACCTTTTGC
TAACAAGGAAGCGTGTGTCCCACTTTCTACAACTTTTCCTTTGTCTAAAACAGCAATCGT
GTCGCAGTTCTGGATCGTGCTAAGCCTATGTGCGATCACAACGCTTGTTTTCCCTACCAT
CACATGTTCGAGGGCGTCTTGCACTACACGCTCTGATTGGCTGTCCAAAGCGCTTGTAGC
TTCATCGAGGAGCAAAATTGATGGATTCTTTAAAATCGTTCGGGCGATAGCTATCCTCTG
TTTTTGACCACCAGACAATTGTACTCCTCTGTCTCCGCAGTACGTATCATAGCCGTCTGA
TAGTGATGTAATGAACTCGTGAGCGTTTGCCGTCTTTCCTGCCTCGATAATCTCCGACTC
GTCGATCTTGTTAGATGCTCGTCCATACATTATGTTTTCTCGGATTGTCCCCGCGAATAA
TGTTGGTTCTTGACTAACCAAAGACATGTGTTGACGAAGTGATCTTAGATGATACGACCT
TATATCACGGCCATCGATTTTCACAATACCTTGTAACGGGTCATAGAACCGTTCGATTAA
CCCGATTACAGTTGACTTACCCGACCGACTTGGACCTACGATCGCTGTCGACTTTCCCTC
GTGGATCTCTATGGAGAAAGTGTTGAATANTACCATATTAGGTCGCGTT
   
Sequence Name 04-K123448-0022-009-C11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K123448-0022-009-C11C-8409
ATTTGTAGCCATTTTTGCCGACTACGCAAAGCCTTATAACGTGCACCTCAGCTTTGAATT
TTTGGTATGGTGGTAAATTAATCGCTGATGGGAAGATGGTATCAAAAGCATTCTTTGAGT
TATTTTTGATCTTTAAGACTACAGGCCGGGCTATTGCCGAAGCTGGGACAATGACGACAG
ATCTAGCCAAGGGCTCAAATTCAGTTGACTCAGTATTCACAGTGTTAGATCGTCGAACCA
CCATTGAGCCGGAGAATCCGGATGGGTATATCTTAGAAAAAATAAAGGGCCAAATCACAT
TTCTCAATGTGGACTTCGCTTACCCAACGCGACCTAATATGGTTATATTCAACAACTTCT
CCATAGAGATCCACGAGGGAAAGTCGACAGCGATCGTAGGTCCAAGTCGGTCGGGTAAGT
CAACTGTAATCGGGTTAATCGAACGGTTCTATGACCCGTTACAAGGTATTGTGAAAATCG
ATGGCCGTGATATAAGGTCGTATCATCTAAGATCACTTCGTCAACACATGTCTTTGGTTA
GTCAAGAACCAACATTATTCGCGGGGACAATCCGAGAAAACATAATGTATGGACGAGCAT
CTAACAAGATCGACGAGTCGGAGATTATCGAGGCAGGAAAGACGGCAAACGCTCACGAGT
TCATTACATCACTATCAGACGGCTATGATACGTACTGCGGAGACAGAGGAGTACAATTGT
CTGGTGGTCAAAAACAGAGGATAGCTATCGCCCGAACGATTTTAAAGAATCCATCAATTT
TGCTCCTCGATGAAGCTACAAGCGCTTTGGACAGCCAATCAGAGCGTGTAGTGCAAGACG
CCCTCGAACATGTGATGGTAGGGAAAACAAGCGTTGTGATCGCACATAGGCTTAGCACGA
TCCAGAACTGCGACACGATTGCTGTTTTAGACAAAGGAAAGTTGTAGAAAGTGGGACACA
CGCTTCCCTGTTAGCAAAAGGTCC

Last Updated on 06.12.2018 16:11