Sequences from confirmation amplicon


Confirmation sequences for line 009E05 and hit At1g31310 are shown.
Line ID 009E05
Locus At1g31310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11199754
Primers used for PCR HV41 - 8474, show primer details
   
Sequence Name 68-K033097-022-009-E05C-8409
GenBank Accession LN511940 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K033097-022-009-E05C-8409
CTTTACATTGAATATATCCTGAGAGACACAAAAAAGTTAGAGAGTACGAGAGACGAAGGG
TAGAATCGTCTATTACCGCCGGTGAGTCGTCTTCATCTTCAGCTCCAGCTGGAGAAACGG
CGTCGTACTGGAAGATGGAGAAGAGTGAGAGGAAAGAGAGAAGCTTACCAAGTAATATGT
TACCTCAAACATACCAAGCTTTGTTTGAAGTTGTTGAGAGTAAAACGCTTCCTTCTTCTA
CCGCCGTAACCGCTGTAACCGCTGCAGTTGCGGCTGCTGCTGCTGCTATTAGTAGTGGAA
ACGGTTCAGGCGGTGGACAAATCCAAAAAGTGATACAACAAGGTTTAGGATTTGTTGTTC
CTAAAGTACATCAAATCATTCAGCAGCAACCTGTTTTGTATACCACTT
   
Sequence Name 20-K033097-022-009-E05C-HV41
GenBank Accession LN495113 [GenBank]
Sequencing primer gene specific primer, HV41: 5'-GAAGTGGTAACAAAACAGGTTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K033097-022-009-E05C-HV41
ACAATCCTAACCTTGTTGTATCACTTTTTGGATTTGTCCACCGCCTGAACCGTTTCCACT
ACTAATAGCAGCAGCAGCAGCCGCAACTGCAGCGGTTACAGCGGTTACGGCGGTAGAAGA
AGGAAGCGTTTTACTCTCAACAACTTCAAACAAAGCTTGGTATGTTTGAGGTAACATATT
ACTTGGTAAGCTTCTCTCTTTCCTCTCACTCTTCTCCATCTTCCAGTACGACGCCGTTTC
TCCAGCTGGAGCTGAAGATGAAGACGACTCACCGGCGGTAATAGACGATTCTACCCTTCG
TCTCTCGTACTCTCTAACTTTTTTGTAGTCTCTCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11