Sequences from confirmation amplicon


Confirmation sequences for line 009H03 and hit At3g52900 are shown.
Line ID 009H03
Locus At3g52900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19613210
Primers used for PCR 01jx - 8474, show primer details
   
Sequence Name 63-K113379-0022-009-H03C-01jx
GenBank Accession LN511029 [GenBank]
Sequencing primer gene specific primer, 01jx: 5'-TCTTGTTGATTTCCTCCAGTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K113379-0022-009-H03C-01jx
CAAAGAAATCTCGTCACAAATCACACATTGGATCTTCAGTAGAAATTTTACAAAGCTTTT
AAGTGGTTTAGACTAAATACCTCCATTAACATGGAAACAAGGTGAGCTTTCTCTTTGTTC
TTTTCATTGAAAGCTTCCAATGTGTCTTTGTATTCCTTCTCCTGTCAAATTATCCTCTTG
TGTTAAAACAAAGCTGCCAGCTTTTCCTGAAAATGAAAATGTGTAATACCTTTTTCTGAC
AGCTTTGTGCCAAAGATTTGATATCTCGATTCGCCATGTCGATTTTCTTCCTCACCATAC
CAACTTCTTTTCTCATAGGATCTCCCATAATCTCTAGCTCCTGTTTTAGTAAGAACATTA
TTAGCTTTGTTGTCTACCATGTTACACAAAAGGCTTTTTATAAACTTACTTCCAATGTTT
GAGTTAAGCATCTTGTAGCTTCCTCTGCAAAACCGAGCCGCTGTTGAACTTTTTCCTTAA
CAGTCATCTTCTTCCTCTCGATCTCTTCTTCTCGGGCTTGAAACCTCGTCACTGCAACTT
TCCATATCTCTTCTTCTTCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCC
   
Sequence Name 25-K113377-0022-009-H03C-8409
GenBank Accession LN497528 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K113377-0022-009-H03C-8409
GCATTTCATGAAGAAGAGAGATATGGAAAGTTGCAGTGACGAGGTTTCAAGCCCGAGAAG
AAGAGATCGAGAGGAAGAAGATGACTGTTAAGGAAAAAGTTCAACAGCGGCTCGGTTTTG
CAGAGGAAGCTACAAGATGCTTAACTCAAACATTGGAAGTAAGTTTATAAAAAGCCTTTT
GTGTAACATGGTAGACAACAAAGCTAATAATGTTCTTACTAAAACAGGAGCTAGAGATTA
TGGGAGATCCTATGAGAAAAGAAGTTGGTATGGTGAGGAAGAAAATCGACATGGCGAATC
GAGATATCAAATCTTTGGCACAAAGCTGTCAGAAAAAGGTATTACACATTTTCATTTTCA
GGAAAAGCTGGCAGCTTTGTTTTAACACAAGAGGATAATTTGACAGGAGAAGGAATACAA
AGACACATTGGAAGCTTTCAATGAAAAGAACAAAGAGAAAGCTCACCTTGTTTCCATGTT
AATGGAGGTATTTAGTCTAAACCACTTAAAAGCTTTGTAAAATTTCTACTGAAGATCCAA
TGTGTGATTTGTGACGATGATTTTCTTTTGGCAGCTACTGGCTGAAAGTGAAAGATTAAG
GATAAAGAAACTGGAGGAATCCAACA

Last Updated on 06.12.2018 16:11