Sequences from confirmation amplicon


Confirmation sequences for line 009H05 and hit At2g40310 are shown.
Line ID 009H05
Locus At2g40310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16834362
Primers used for PCR 14mk - 8474, show primer details
   
Sequence Name 39-K114225-0022-009-H05C-14mk
GenBank Accession LN503435 [GenBank]
Sequencing primer gene specific primer, 14mk: 5'-ATCACCGGAAGCTGTTATAGTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114225-0022-009-H05C-14mk
TGAATGTCCCATTTTGCTGTTGAGTGATGTTATGTCTTTGATATTTGAGTTGTTCACGAA
TGCAAATCCCATAGTCTATAAAAAAATTCAAGTTTATTATAAGACTTGTTAATTTCTCAA
ATGAAAAAGACCAAAAATTCTAATAAAGTTGTGTATAAATATCACATGTACGTTTTGTAA
TACGCTTTAAATGTAAATAAGTGAAAAAGTTTAAGATATGTTACCATAGCTAGTTTAGGA
CAATTGAGATTTTTGTGGCAGTCATTGAGTGGCCAAGATTGTTTTCCTTGGCCGTCAAGT
GTACCGGAACCGGAGATAGAAAGATTGTTGATGTACCTAAAGTTGATCCATGTATCTTGC
TTAATATCACTAGGATTTGCAGGAGCCAATAAAGTTCCATCAATGACGAATTCAATAGGA
TTTTTGCATGGACCCACAAACTCTACACCACCGAGGTAAAATATTCCTTTTGGTACATAG
ATTTTACTACTTCCACTTTTTCTTTTGCATGCACGACTCCNTATACTTGTGAATACCTGA
TTTTGTATGTGTTAAGAATGATATATTTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 19-K114224-0022-009-H05C-8409
GenBank Accession LN494854 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114224-0022-009-H05C-8409
TTTCAATATATCATTCTTAACCATACAAAATCAGGTATTCACAAGTATATGGAGTCGTGC
ATGCAAAAGAAAAAGTGGAAGTAGTAAAATCTATGTACCAAAAGGAATATTTTACCTCGG
TGGTGTAGAGTTTGTGGGTCCATGCAAAAATCCTATTGAATTCGTCATTGATGGAACTTT
ATTGGCTCCTGCAAATCCTAGTGATATTAAGCAAGATACATGGATCAACTTTAGGTACAT
CAACAATCTTTCTATCTCCGGTTCCGGTACACTTGACGGCCAAGGAAAACAATCTTGGCC
ACTCAATGACTGCCACAAAAATCTCAATTGTCCTAAACTAGCTATGGTAACATATCTNAA
ACTTTTTCACTTATTTACATTTAAAGCGTATTACAAAACGTACATGTGATATTTATACAC
AACTTTATTAGAATTTTTGGTCTTTTTCATTTGAGAAATTAACAAGTCTTATAATAAACT
TGAATTTTTTTATAGACTATGGGATTTGCATTCGTGAACAACTCANATATCANAGACATA
ACATCACTCAACAGCAAAATGGGACATTTCAACTTCTTCTCCGTCCATCACTTTAATATT
ACCGGCGTCACTA

Last Updated on 06.12.2018 16:11