Sequences from confirmation amplicon


Confirmation sequences for line 010C04 and hit At3g59260 are shown.
Line ID 010C04
Locus At3g59260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21904846
Primers used for PCR 02lf - 8474, show primer details
   
Sequence Name 67-K113920-0022-010-C04C-8409
GenBank Accession LN511861 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K113920-0022-010-C04C-8409
ACTCCTTTCTTCCTTATATTGATTTCATTTACACTTGAATATACTCATTGCTCGATCCAA
AAGCTCAAACCCACCAGGCCGTTCCAGAGTCATGGACCGCCTTTGCCTACATTGTAGAAG
GCGATGAAGGCGTTTTTAGCTCCTCGGACTCTTCCACAGTACAAGCACATAATGTTGTGG
TGTTTGGGACAGGGGATGAAGTTAGCGTGTGGAACACGTCAAACTCTAGGCCGTTGAGGT
TCTTGTTGATTGCAGGGGAACCGATAGGCGAGCCTGTGGTTCAGCATGGGCCATTTGTGA
TGAACTCACAGGATGAGATTGAAATGACAATTGGGGACTATCGTAATGGCATGAATGGCT
TCGAAATGGCCAAGCATTGGAGGTCAGAGTGAAGAGTTTGAAGCAATAACCAGAGAGACA
GTTATATTTAGGGTTTCTTATTTATTTCACATGGTTTTTTAATCTTACATTCTATATCAT
ATCATTGTCACGTAATAAAAAAAAATGCTACGTTTTAGAAAATTGATTAACTATAGTTCA
AGCTTATCACTTTCAGATTTAAAGGGTAAATATTTTTCACAAAAGAAAAATCACACTCAT
GGCTTAAAGATAGTAAAAAGAGGCATGAGTGA
   
Sequence Name 57-K113919-0022-010-C04C-02lf
GenBank Accession LN509483 [GenBank]
Sequencing primer gene specific primer, 02lf: 5'-TCACTCATGCCTCTTTTTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K113919-0022-010-C04C-02lf
TATTTACCCTTTAATCTGAAAGTGATAAGCTTGAACTATAGTCTATCAATTTTCTAAAAC
GTAGCATTTTTTTTTATTACGTGACAATGATATGATATAGAATGTAAGATTAAAAAACCA
TGTGAAATAAATAAGAAACCCTAAATATAACTGTCTCTCTGGTTATTGCTTCAAACTCTT
CACTCTGACCTCCAATGCTTGGCCATTTCGAAGCCATTCATGCCATTACGATAGTCCCCA
ATTGTCATTTCAATCTCATCCTGTGAGTTCATCACAAATGGCCCATGCTGAACCACAGGC
TCGCCTATCGGTTCCCCTGCAATCAACAAGAACCTCAACGGCCTAGAGTTTGACGTGTTC
CACACGCTAACTTCATCCCCTGTCCCAAACACCACAACATTATGTGCTTGTACTGTGGAA
GAGTCCGAGGAGCTAAAAACGCCTTCATCGCCTTCTACAATGTAGGCAAAGGCGGTCCAT
GACTCTGGAACGGCCTGGTGGGTTTGAGCTTTTGGATCGAGCAATGAGTATATTCAATTG
TAAATGAAATCAATATAAGGAAGAAAGGAGTATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11