Sequences from confirmation amplicon


Confirmation sequences for line 010C05 and hit At4g28540 are shown.
Line ID 010C05
Locus At4g28540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14110280
Primers used for PCR PC16 - 8474, show primer details
   
Sequence Name 36-K103731-0022-010-C05C-PC16
GenBank Accession LN502197 [GenBank]
Sequencing primer gene specific primer, PC16: 5'-AGGATTTCAGGTAACCCTTGACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K103731-0022-010-C05C-PC16
ATAACTCTTCTTCGCTAACGGTGGTCGGTTCTGATTTTGTGATCCTGTCTATGTCTTACA
GTAGGGAACATCCGCGATAAATTCTCAGGTGCGGTCGAAGCATTTGCGAGAAGGAACGTT
AGAGGACCCAGTCCCCATCAAAACCATACCAGACATCGAACTCTTGACGAAATTCCTTCA
ATGAAACCTGCTGTGGTAAAAAATAATATCATCATCTTATATATATCAAAATTTTGATAT
ATTGTTATTACATTATAGAACGTGCTTAGTGATTTTAACACTTTTCAAGAAATCAATCTT
GCTCGTTCTTGATATATCAATTTATTTGATATTGTCAGAATATGGTATCTGAGAAAGGAA
GAAACACTTCCAGATACGGCAGTGCTTCGAGGAGAGCAGTAGCCTCAGGATGATAGCCTC
AGGATGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 52-K103732-0022-010-C05C-8409
GenBank Accession LN507791 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K103732-0022-010-C05C-8409
CCTGAGGCTATATCCTGAGGCTACTGCTCTCCTCGAAGCACTGCCGTATCTGGAAGTGTT
TCTTCCTTTCTCAGATACCATATTCTGACAATATCAAATAAATTGATATATCAAGAACGA
GCAAGATTGATTTCTTGAAAAGTGTTAAAATCACTAAGCACGTTCTATAATGTAATAACA
ATATATCAAAATTTTGATATATATAAGATGATGATATTATTTTTTACCACAGCAGGTTTC
ATTGAAGGAATTTCGTCAAGAGTTCGATGTCTGGTATGGTTTTGATGGGGACTGGGTCCT
CTAACGTTCCTTCTCGCAAATGCTTCGACCGCACCTGAGAATTTATCGCGGATGTTCCCT
ACTGTAAGACATAGACAGGATCACAAAATCAGAACCGACCACCGTTTAGCGAAGAAGAGT
TATATGATTCTTAACTGTAGAAAAAAGTCAAGGGTTACCGGAAATC

Last Updated on 06.12.2018 16:11