Sequences from confirmation amplicon


Confirmation sequences for line 010E02 and hit At1g11940 are shown.
Line ID 010E02
Locus At1g11940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4033644
Primers used for PCR 01nt - 8474, show primer details
   
Sequence Name 65-K114626-0022-010-E02C-01nt
GenBank Accession LN511456 [GenBank]
Sequencing primer gene specific primer, 01nt: 5'-ACAAAACTCCTCGGTGAAGAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114626-0022-010-E02C-01nt
CCAGTCTATATGGAGCACATCTGCAAACAGAAAGTAGATGAAAATGAAATAAGTCCGTAC
AAACCACAAATGTGCTCAAAGTCAGATGCAATAAGTGGTCAGGCATAAGATGATCTGAAA
GAGTAATGCTGAAAGAAGCAAACCTGTCAGAGAGAAGAACAAATCTTTGATTGGAGTGAT
CCTCTAAAGCAGATGCAAGCAACAATCTTTCTGCTTCAATCATGCTTGATTCTCCCCATA
CTACCTATAACCATAACAACACCACCCTAAGTTTCACTATAAAAAAACATCAAATGGAGA
AATTCTCATGAACACTCACCTTAATGCTATTGTTCAATTGGCGATTATAGAAATACTGTG
ATCGTGTAGTTTCTTCATTGAACACGAAACCAGGTACTGAATGTATATAAATTGAGAAAT
TCGCATGATCCACTCCCTGCAAAATCACCAGAATCAGCAATTTTTTCGAAATTTAGAATA
ACGGACAAGTATTAATAGGACTAAAATGGTTTAACAGGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 65-K114625-0022-010-E02C-8409
GenBank Accession LN511455 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114625-0022-010-E02C-8409
GCCATTTCATTGAATATATCCTGTTAACCATTTTAGTCCTATTAATACTTGTCCGTTATT
CTAAATTTCGAAAAAATTGCTGATTCTGGTGATTTTGCAGGGAGTGGATCATGCGAATTT
CTCAATTTATATACATTCAGTACCTGGTTTCGTGTTCAATGAAGAAACTACACGATCACA
GTATTTCTATAATCGCCAATTGAACAATAGCATTAAGGTGAGTGTTCATGAGAATTTCTC
CATTTGATGTTTTTTTATAGTGAAACTTAGGGTGGTGTTGTTATGGTTATAGGTAGTATG
GGGAGAATCNAGCATGATTGAAGCAGAAAGATTGTTGCTTGCATCTGCTTTAGAGGATCA
CTCCAATCAAAGATTTGTTCTTCTCTCTGACAGGTTTGCTTCTTTCAGCATTACTCTTTC
AGATCATCTTATGCCTGACCACTTATTGCATCTGACTTTGAGCACATTTGTGGTTTGTAC
GGACTTATTTCATTTTCATCTACTTTCTGTTTTGCAGATGTGCTCCATTATATGACTTTG
GCTATATATACAAATATCTTATCTCTTCACCGAGGAGTTTTG

Last Updated on 06.12.2018 16:11